A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants
| Autor: | Ludovica Curcio, Marcella Ciullo, Martina Torricelli, Cristina Pesca, Andrea Felici, Martina Sebastianelli, M. Biagetti, C. Sebastiani, Deborah Cruciani, Silvia Crotti |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Salmonella 040301 veterinary sciences 030106 microbiology Brucella Abortion Septic medicine.disease_cause Real-Time Polymerase Chain Reaction Microbiology Sensitivity and Specificity 0403 veterinary science 03 medical and health sciences Leptospira Pregnancy medicine Animals Mass Screening Pregnancy Complications Infectious Molecular Biology Chlamydia biology Toxoplasma gondii 04 agricultural and veterinary sciences Ruminants Coxiella burnetii biology.organism_classification medicine.disease Virology Neospora caninum Molecular Diagnostic Techniques Identification (biology) Female Multiplex Polymerase Chain Reaction |
| Zdroj: | Journal of microbiological methods. 148 |
| ISSN: | 1872-8359 |
| Popis: | Abortion in ruminants represents an important economic concern for farmers. Microbial agents, such as Brucella spp., Chlamydia spp., Coxiella burnetii , Leptospira spp., Neospora caninum , Salmonella spp. and Toxoplasma gondii , are among the main infectious causes of abortion and require rapid and reliable diagnosis. This study describes the development of a multi-screening assay using Fast Real-Time PCR (Fast qPCR) that allows, in a single test, the simultaneous identification of the above-mentioned abortive agents. This multi-screening approach is characterized by a mean diagnostic sensitivity and specificity of 100% and 97%, respectively; it has a limit of detection (LOD) ranging from 5 × 10 3 to 4 × 104 genomic copies/g of tissue and a very good concordance with traditional end-point PCR assays used in routine diagnostic activity. The proposed method represents a rapid approach to the simultaneous detection of the main abortive agents in ruminants that allows to make an accurate diagnosis and to set up appropriate control measures in a short period of time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect