Involvement of the Tubular ClC-Type Exchanger ClC-5 in Glomeruli of human Proteinuric Nephropathies
| Autor: | Angela D'Angelo, Mila Della Barbera, Dorella Del Prete, Luciana Bonfante, Emilia Tiralongo, Daniela Vianello, Franca Anglani, Annunziata Marangelli, Hans J. Baelde, Giovanni Betto, Monica Ceol, Marialuisa Valente |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Protein Structure Anatomy and Physiology medicine.medical_treatment Kidney Glomerulus lcsh:Medicine Gene Expression Histopathology Biology Endocytosis Real-Time Polymerase Chain Reaction Nephropathy Diagnostic Medicine Chloride Channels Biopsy Molecular Cell Biology medicine Macromolecular Structure Analysis Humans RNA Messenger lcsh:Science Renal Physiology Multidisciplinary medicine.diagnostic_test urogenital system lcsh:R Albumin Computational Biology Renal System medicine.disease Nephrectomy Proteinuria Real-time polymerase chain reaction Kidney Tubules Anatomical Pathology Nephrology Immunohistochemistry Medicine lcsh:Q Kidney Diseases Nephrotic syndrome Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 9, p e45605 (2012) |
| Popis: | Glomerular protein handling mechanisms have received much attention in studies of nephrotic syndrome. Histopathological findings in renal biopsies from severely proteinuric patients support the likelihood of protein endocytosis by podocytes. ClC-5 is involved in the endocytosis of albumin in the proximal tubule. Aim To investigate whether ClC-5 is expressed in the glomerular compartment and whether it has a role in proteinuric nephropathies. ClC-5 expression was studied using Real-time PCR in manually- and laser-microdissected biopsies from patients with type 2 diabetes (n 37) and IgA nephropathy (n 10); in biopsies of membranous glomerulopathy (MG) (n 14) immunohistochemistry for ClC-5 (with morphometric analysis) and for WT1 was done. Controls: cortical tissue (n 23) obtained from unaffected parts of tumor-related nephrectomy specimens. Results ClC-5 was expressed at glomerular level in all biopsies. Glomerular ClC-5 levels were significantly higher in diabetic nephropaty and MG at both mRNA and protein level (p |
| Databáze: | OpenAIRE |
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