Genome-Wide DNA Methylation Profile of Genecis-Acting Element Methylations in All-transRetinoic Acid-Induced Mouse Cleft Palate
| Autor: | Shenyou Shu, Zhan Ouyang, Lin Zhu, Xuan Shu, Yuxia Zhai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Retinoic acid Intron Cell Biology General Medicine Methylation 030105 genetics & heredity Biology Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry DNA methylation Gene expression Genetics Enhancer Molecular Biology Gene DNA |
| Zdroj: | DNA and Cell Biology. 37:993-1002 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2018.4369 |
| Popis: | DNA methylation epigenetically regulates gene expression. This study is aimed to investigate genome-wide DNA methylations involved in the regulation of palatal fusion in the all-trans retinoic acid-induced mouse cleft palate model. There were 4,718,556 differentially CCGG methylated sites and 367,504 CCWGG methylated sites for 1497 genes between case and control embryonic mouse palatal tissues. The enhancers (HDAC4 and SMAD3) and promoter (MID1) of these three genes had cis-acting element methylation. HDAC4 is localized within the CCWGG, while MID1 and SMAD3 are localized within the CCGG of the gene intron. The methylation-specific polymerase chain reaction data confirmed the MethylRAD-seq results, while the quantitative reverse transcriptase-polymerase chain reaction result showed that changes in gene expression inversely were associated with the cis-acting element methylation of the gene during retinoic acid-induced palatal fusion. The GO and KEGG data showed that these three genes could regulate cell proliferation, skeletal muscle fiber development, and development-related gene signaling or activity. The cis-acting element methylation of HDAC4, SMAD3, and MID1 may play a regulatory role during palatal fusion. Further research is needed to verify these novel epigenetic biomarkers for cleft palate. |
| Databáze: | OpenAIRE |
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