Portland cement for bone tissue engineering: Effects of processing and metakaolin blends
Autor: | Luis E. López, Daniel Gallego-Perez, Olga M. Posada, Natalia Higuita-Castro, Felipe Garcia Quiroz, Alan S. Litsky, Derek J. Hansford |
---|---|
Rok vydání: | 2010 |
Předmět: |
Cement
Materials science Osteoblasts Tissue Engineering Tissue Scaffolds Scanning electron microscope Construction Materials Simulated body fluid Biomedical Engineering Energy-dispersive X-ray spectroscopy Bone Cements Bone and Bones law.invention Biomaterials Portland cement law Materials Testing Slurry Humans Composite material Electron microscope Kaolin Metakaolin |
Zdroj: | Journal of biomedical materials research. Part B, Applied biomaterials. 98(2) |
ISSN: | 1552-4981 |
Popis: | The need for a suitable scaffolding material for load bearing bone tissue engineering still has yet to be met satis- factorily. In this study, Portland cement and Portland cement/ metakaolin (MK) blends were processed to render them bio- logically and mechanically suitable for such application. Port- land cement was mixed with MK at different ratios. The slurries were hydrated under atmospheric (noncarbonated samples) and high-CO2 conditions (carbonated samples). The mechanical properties were characterized via compressive tests. The bioactivity was analyzed in a simulated body fluid solution. Scanning electron microscopy and energy dispersive spectroscopy were used to evaluate sample morphology and chemistry. The cytocompatibility (direct contact assay, MTT test, and alkaline phosphatase activity) was tested using human osteoblast-like cells. Cell responses were observed via conventional and electron microscopy. The results showed that the implementation of MK did not significantly influence the mechanical properties. All the samples evidenced bioac- tive behavior. Cell experiments confirmed a highly cytotoxic response to the noncarbonated specimens. The introduction of MK as well as the CO2 pretreatment significantly improved the cytocompatibility of the specimens. These results show that properly processed Portland cement and Portland cement/MK blends could present suitable properties for the development of load-bearing scaffolding structures in bone tissue-engineering applications. V C 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 98B: 308-315, 2011. |
Databáze: | OpenAIRE |
Externí odkaz: |