The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System
| Autor: | Ekta Dembla, Silke Wahl, Frank Schmitz, Marie-Lisa Eich, Mayur Dembla, Karin Schwarz |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
retina splice variant Endocytic cycle Ribbon synapse Biology lcsh:RC321-571 darkness-induced synaptic recruitment of dynamin1xb Synapse 03 medical and health sciences Cellular and Molecular Neuroscience synapse medicine splice calcineurin lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Molecular Biology Original Research Synaptic ribbon Retina Synaptic pharmacology dynamin1xb Cell biology 030104 developmental biology medicine.anatomical_structure Synaptic plasticity sense organs Neuroscience |
| Zdroj: | Frontiers in Molecular Neuroscience, Vol 10 (2017) Frontiers in Molecular Neuroscience |
| ISSN: | 1662-5099 |
| Popis: | In the present study, we generated and characterized a splice site-specific monoclonal antibody that selectively detects the calcineurin-binding dynamin1 splice variant dynamin1xb. Calcineurin is a Ca2+-regulated phosphatase that enhances dynamin1 activity and is an important Ca2+-sensing mediator of homeostatic synaptic plasticity in neurons. Using this dynamin1xb-specific antibody, we found dynamin1xb highly enriched in synapses of all analyzed brain regions. In photoreceptor ribbon synapses, dynamin1xb was enriched in close vicinity to the synaptic ribbon in a manner indicative of a peri-active zone immunolabelling. Interestingly, in dark-adapted mice we observed an enhanced and selective enrichment of dynamin1xb in both synaptic layers of the retina in comparison to light-adapted mice. This could be due to an illumination-dependent recruitment of dynamin1xb to retinal synapses and/or due to a darkness-induced increase of dynamin1xb biosynthesis. These latter findings indicate that dynamin1xb is part of a versatile and highly adjustable, activity-regulated endocytic synaptic machinery. |
| Databáze: | OpenAIRE |
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