Effect of serum from mice immunized with group A streptococcus antigens on the efficiency of in vitro cloning of stromal precursor cells
| Autor: | V. I. Grabko, N. E. Sharapova, T. A. Danilova, U. F. Gorskaya, Vladimir G. Lunin, V. G. Nesterenko |
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| Rok vydání: | 2009 |
| Předmět: |
Serum
Stromal cell Streptococcus pyogenes Guinea Pigs Bone Marrow Cells Biology medicine.disease_cause Group A General Biochemistry Genetics and Molecular Biology Interferon-gamma Mice Immune system Antigen Precursor cell medicine Animals Cells Cultured Antigens Bacterial Streptococcus Tumor Necrosis Factor-alpha Granulocyte-Macrophage Colony-Stimulating Factor General Medicine Molecular biology Interleukin-12 In vitro Clone Cells Interleukin-10 medicine.anatomical_structure Immunology Mice Inbred CBA Interleukin-2 Bone marrow Interleukin-4 Interleukin-5 Stromal Cells |
| Zdroj: | Bulletin of experimental biology and medicine. 146(6) |
| ISSN: | 1573-8221 |
| Popis: | The efficiency of cloning of stromal precursor cell in mouse bone marrow culture increases significantly (2-3-fold) in the presence of serum from mice immunized with type 5 group A streptococcus antigens (5-20 microl serum/ml culture medium) in comparison with intact animal serum. The levels of TNF-alpha and IFN-gamma are significantly reduced (2.7 times and more than 6-fold, respectively) in the sera of immunized mice in comparison with normal serum. Serum levels of IL-2, -4, -5, -10, and -12 were about the same in both groups; no granulocyte-macrophage CSF was detected. These data attest to appreciable effect of immunization with streptococcal antigens on the bone marrow stromal tissue; this effect is presumably mediated through serum cytokines. |
| Databáze: | OpenAIRE |
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