Differential regulation of uncoupling protein-1, -2 and -3 gene expression by sympathetic innervation in brown adipose tissue of thermoneutral or cold-exposed rats
| Autor: | Alain Géloën, Hervé Barré, F. Cohen-Adad, Joël Lachuer, Frédérique Denjean, Claude Duchamp, Colette Moulin |
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| Jazyk: | angličtina |
| Předmět: |
Male
medicine.medical_specialty Sympathetic Nervous System medicine.medical_treatment Cold exposure Biophysics Adipose tissue Biology Biochemistry Unilateral sympathetic denervation Ion Channels Mitochondrial Proteins Adipose Tissue Brown Structural Biology Internal medicine Brown adipose tissue Gene expression Genetics medicine Animals Uncoupling Protein 3 Uncoupling Protein 2 Northern blot RNA Messenger Rats Wistar Sympathectomy Semi-quantitative reverse transcriptase polymerase chain reaction Molecular Biology Uncoupling Protein 1 Messenger RNA Analysis of Variance Temperature Membrane Proteins Membrane Transport Proteins Proteins Cell Biology Thermogenin Rats Cold Temperature medicine.anatomical_structure Endocrinology Gene Expression Regulation Sympathetic innervation Carrier Proteins |
| Zdroj: | FEBS Letters. (2-3):181-185 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(99)00056-3 |
| Popis: | The control of uncoupling protein-1, -2 and -3 (UCP-1, UCP-2, UCP-3) mRNA levels by sympathetic innervation in rats was investigated by specific and sensitive RT-PCR assays. In rats reared at thermoneutrality (25 degrees C), unilateral surgical sympathetic denervation of interscapular brown adipose tissue (BAT) markedly reduced the UCP-1 mRNA level (-38%) as compared with the contralateral innervated BAT pad, but was without significant effect on UCP-2 and -3 mRNA levels. Cold exposure (7 days, 4 degrees C) markedly increased UCP-1 (+180%), UCP-2 (+115%) and UCP-3 (+195%) mRNA levels in interscapular BAT. Unilateral sympathetic denervation prevented the cold-induced rise in BAT UCP-1 and UCP-2 mRNAs, but not that in BAT UCP-3 mRNA. Results were confirmed by Northern blot analysis. These data indicate a differential endocrine control of UCP-1, UCP-2 and UCP-3 gene expression in rat BAT both at thermoneutrality and during prolonged cold exposure. |
| Databáze: | OpenAIRE |
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