Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages

Autor: Xue-Jun Fan, Shanshan Liang, Ruoyu Wang, Heming Li, Xi Chen, Shi-Chao Han, Xuening Ji, Xiu-lian Quan, Jin-Ming Zhu
Rok vydání: 2018
Předmět:
Vascular Endothelial Growth Factor A
0301 basic medicine
Article Subject
Immunocytochemistry
Models
Biological
Metastasis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Stomach Neoplasms
Cell Line
Tumor
Proliferating Cell Nuclear Antigen
Spheroids
Cellular
Tumor Microenvironment
medicine
Humans
Lactic Acid
lcsh:RC799-869
Cell Proliferation
Tumor microenvironment
Hepatology
biology
Cell growth
business.industry
Macrophages
Carcinoma
Gastroenterology
General Medicine
medicine.disease
Molecular biology
Coculture Techniques
Proliferating cell nuclear antigen
Vascular endothelial growth factor
Vascular endothelial growth factor A
Glucose
030104 developmental biology
Matrix Metalloproteinase 9
chemistry
Cell culture
030220 oncology & carcinogenesis
biology.protein
Matrix Metalloproteinase 2
lcsh:Diseases of the digestive system. Gastroenterology
business
Research Article
Zdroj: Canadian Journal of Gastroenterology & Hepatology
Canadian Journal of Gastroenterology and Hepatology, Vol 2018 (2018)
ISSN: 2291-2797
2291-2789
Popis: The important factors of poor survival of gastric cancer (GC) are relapse and metastasis. For further elucidation of the mechanism, a culture system mimicking the microenvironment of the tumor in humans was needed. We established a model of microencapsulated SGC7901 human GC cells and evaluated the effects of coculturing spheres with tumor-associated macrophages (TAMs). SGC7901 cells were encapsulated in alginate-polylysine-sodium alginate (APA) microcapsules using an electrostatic droplet generator. MTT assays showed that the numbers of microencapsulated cells were the highest after culturing for 14 days. Metabolic curves showed consumption of glucose and production of lactic acid by day 20. Immunocytochemistry confirmed that Proliferating Cell Nuclear Antigen (PCNA) and Vascular Endothelial Growth Factor (VEGF) were expressed in microencapsulated SGC7901 cells on days 7 and 14. The expression of PCNA was observed outside spheroids; however, VEGF was found in the entire spheroids. PCNA and VEGF were increased after being cocultured with TAMs. Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions were detected in the supernatant of microencapsulated cells cocultured with TAMs but not in microencapsulated cells. Our study confirms the successful establishment of the microencapsulated GC cells. TAMs can promote PCNA, VEGF, MMP-2, and MMP-9 expressions of the GC cells.
Databáze: OpenAIRE
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