Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Cystic Fibrosis Patients from Argentina
| Autor: | M. Vazquez, S. Lubovich, L. Galanternik, Paula Pena Amaya, Marta Mollerach, María Sol Haim, Alejandro Teper, Sabrina Di Gregorio, Silvina Fernández |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Methicillin-Resistant Staphylococcus aureus 0301 basic medicine Microbiology (medical) CIENCIAS MÉDICAS Y DE LA SALUD Adolescent Cystic Fibrosis 030106 microbiology Immunology Inmunología Argentina Erythromycin Microbial Sensitivity Tests Multidrug resistance medicine.disease_cause Microbiology Cystic fibrosis 03 medical and health sciences medicine Humans Prospective Studies Child Genotyping Pharmacology Molecular Epidemiology MRSA-ST5-IV MRSA-ST30-IV Molecular epidemiology business.industry SCCmec Infant Newborn Infant Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Medicina Básica Staphylococcus aureus Child Preschool Multilocus sequence typing Female Gentamicin business Multilocus Sequence Typing medicine.drug |
| Zdroj: | Microbial Drug Resistance. 24:613-620 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Methicillin-resistant Staphylococcus aureus (MRSA) colonization in cystic fibrosis (CF) patients is an increasing problem in many countries. In our Respiratory Center at the Hospital de Nin˜os ‘‘Dr. Ricardo Gutie´rrez’’, Buenos Aires, Argentina, the prevalence has climbed from 23% in 1995 up to 32% in 2011. Our objective was to analyze the diversity of MRSA isolates recovered from respiratory samples of CF patients attending our center, characterizing their phenotypes and clonal distribution. Therefore, a prospective study was conducted on all CF patients attending the pediatric Respiratory Center between June 2012 and May 2013 to collect MRSA isolates. Antibiotic susceptibility testing, multilocus sequence typing, pulsed-field gel electrophoresis, spa typing, and agr genotyping were performed on collected isolates. The prevalence of MRSA during this period was 34.2%, and 71.9% of the patients were infected with isolates that carried SCCmec IV. High resistance rates were detected for gentamicin, erythromycin, clindamycin, ciprofloxacin, and rifampicin. Strains related to the communityassociated MRSA clones, ST5-IV and ST30-IV, were the most frequently recovered. Remarkably, even though most of the isolates were related to these clones, the rate of multi-resistance shown in CF patients was higher than that reported for the same lineages recovered from other infections in our country. Fil: Pena Amaya, Paula. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Haim, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Di Gregorio, Sabrina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Teper, Alejandro Manuel. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Vázquez, Miryam. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Lubovich, Silvina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Galanternik, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Mollerach, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina |
| Databáze: | OpenAIRE |
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