Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis
| Autor: | Hui-Liang Li, Shi-Qing Peng, Di-Feng Zhan, Jia-Hong Zhu, Dong Guo, Ying Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine natural rubber Plant Science lcsh:Plant culture complex mixtures 01 natural sciences Transcriptome 03 medical and health sciences laticifer lcsh:SB1-1110 MYB Gene Transcription factor Genetics MYB-type transcription factor biology technology industry and agriculture Promoter biology.organism_classification body regions Hevea brasiliensis 030104 developmental biology Laticifer biosynthesis Transcription Factor Gene 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 8 (2017) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2017.01974 |
| Popis: | MYB transcription factors hold vital roles in the regulation of plant secondary metabolic pathways. Laticifers in rubber trees (Hevea brasiliensis) are of primary importance in natural rubber production because natural rubber is formed and stored within these structures. To understand the role of MYB transcription factors in the specialized cells, we identified 44 MYB genes (named HblMYB1 to HblMYB44) by using our previously obtained transcriptome database of rubber tree laticifer cells and the public rubber tree genome database. Expression profiles showed that five MYB genes were highly expressed in the laticifers. HblMYB19 and HblMYB44 were selected for further study. HblMYB19 and HblMYB44 bound the promoters of HbFDPS1, HbSRPP, and HRT1 in yeast. Furthermore, the transient overexpression of HblMYB19 and HblMYB44 in tobacco plants significantly increased the activity of the promoters of HbFDPS1, HbSRPP, and HRT1. Basing on this information, we proposed that HblMYB19 and HblMYB44 are the regulators of HbFDPS1, HbSRPP, and HRT1, which are involved in the biosynthesis pathway of natural rubber. |
| Databáze: | OpenAIRE |
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