Profiling serum antibodies with a pan allergen phage library identifies key wheat allergy epitopes
| Autor: | Pamela A. Frischmeyer-Guerrerio, Anna Nowak-Wegrzyn, Audrey C. Knight, Daniel R. Monaco, Thomas R. Nirschl, Brandon M. Sie, Robert A. Wood, H. Benjamin Larman, Robert G. Hamilton, Hugh A. Sampson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male Phage display Adolescent Science General Physics and Astronomy Wheat Hypersensitivity Immunoglobulin E medicine.disease_cause General Biochemistry Genetics and Molecular Biology Epitope Article Antibodies 03 medical and health sciences Epitopes Young Adult 0302 clinical medicine Allergen Antigen Peptide Library medicine Hypersensitivity Humans Peptide library Child Plant Proteins Multidisciplinary biology food and beverages General Chemistry Translational research Allergens Middle Aged medicine.disease 030104 developmental biology 030228 respiratory system Child Preschool Immunoglobulin G Immunology biology.protein Mucosal immunology Female Antibody Wheat allergy Software Antimicrobial Cationic Peptides |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Allergic reactions occur when IgE molecules become crosslinked by antigens such as food proteins. Here we create the ‘AllerScan’ programmable phage display system to characterize the binding specificities of anti-allergen IgG and IgE antibodies in serum against thousands of allergenic proteins from hundreds of organisms at peptide resolution. Using AllerScan, we identify robust anti-wheat IgE reactivities in wheat allergic individuals but not in wheat-sensitized individuals. Meanwhile, a key wheat epitope in alpha purothionin elicits dominant IgE responses among allergic patients, and frequent IgG responses among sensitized and non-allergic patients. A double-blind, placebo-controlled trial shows that alpha purothionin reactivity, among others, is strongly modulated by oral immunotherapy in tolerized individuals. AllerScan may thus serve as a high-throughput platform for unbiased analysis of anti-allergen antibody specificities. A diverse array of antigens can trigger allergic reactions. Here the authors present the ‘AllerScan’ programmable phage display library, which is an efficient and unbiased approach for profiling anti-allergen antibody reactivities at cohort scale, with which a key wheat epitope is found to distinguish between wheat allergy and tolerance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|