Severe burn increased skeletal muscle loss in mdx mutant mice
| Autor: | Melody R. Saeman, Ming Mei Liu, Juquan Song, Steven E. Wolf, Kevin Despain |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Male medicine.medical_specialty Duchenne muscular dystrophy Blotting Western Isometric exercise MyoD Real-Time Polymerase Chain Reaction Article 03 medical and health sciences Mice 0302 clinical medicine Atrophy Internal medicine medicine Myocyte Animals Muscle Skeletal Tissue homeostasis Myogenin Trauma Severity Indices business.industry Skeletal muscle 030208 emergency & critical care medicine Anatomy musculoskeletal system medicine.disease Muscular Dystrophy Duchenne Muscular Atrophy 030104 developmental biology Endocrinology medicine.anatomical_structure Body Composition Mice Inbred mdx Surgery business Burns tissues Biomarkers |
| Zdroj: | The Journal of surgical research. 202(2) |
| ISSN: | 1095-8673 |
| Popis: | Background Severe burn causes muscle mass loss and atrophy. The balance between muscle cell death and growth maintains tissue homeostasis. We hypothesize that preexisting cellular structural defects will exacerbate skeletal muscle mass loss after burn. Using a Duchenne muscular dystrophy (mdx) mutant mouse, we investigated whether severe burn caused more damage in skeletal muscle with preexisting muscle disease. Methods The mdx mice and wild-type (WT) mice received 25% total body surface area scald burn. Gastrocnemius (GM), tibialis anterior, and gluteus muscles were obtained at days 1 and 3 after burn. GM muscle function was measured on day 3. Animals without burn served as controls. Results Wet tissue weight significantly decreased in tibialis anterior and gluteus in both mdx and WT mice after burn (P |
| Databáze: | OpenAIRE |
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