Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD
| Autor: | Dongho Lee, Mi Hee Woo, Eun Sook Ma, Eun Kyoung Seo, Je-Hyun Lee, Dong Cheul Moon, Kyoung Hwangbo, Su Yang Jeong, Byung Sun Min, Jong Keun Son, Bing Tian Zhao |
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| Rok vydání: | 2013 |
| Předmět: |
Light
Analytical chemistry High-performance liquid chromatography chemistry.chemical_compound Betaine Chromatography detector Pattern recognition Drug Discovery Scattering Radiation Acetonitrile Chromatography High Pressure Liquid Chromatography Isocratic elution Hydrophilic interaction chromatography Organic Chemistry Quality control Lycium ELSD chemistry Fruit Molecular Medicine Lycii Fructus HPLC Ammonium acetate Quantitative analysis (chemistry) Research Article |
| Zdroj: | Archives of Pharmacal Research |
| ISSN: | 1976-3786 0253-6269 |
| Popis: | A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus. |
| Databáze: | OpenAIRE |
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