RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge
| Autor: | Jyothi Thimmapuram, Taylor W. Bailey, Andrea Pires dos Santos, Naíla C. do Nascimento, Abigail Cox, M. Preeti Sivasankar, Shaojun Xie |
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| Rok vydání: | 2020 |
| Předmět: |
Male
lcsh:QH426-470 lcsh:Biotechnology RNA-Seq Vocal Cords Biology Proteomics Transcriptome 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation lcsh:TP248.13-248.65 In vivo Gene expression Genetics Animals Animal model 030223 otorhinolaryngology Gene 030304 developmental biology 0303 health sciences Dehydration Sequence Analysis RNA Gene Expression Profiling Vocal folds RNA Humidity Fold change Cell biology Airway lcsh:Genetics Rabbits Larynx Research Article Biotechnology |
| Zdroj: | BMC Genomics, Vol 21, Iss 1, Pp 1-13 (2020) BMC Genomics |
| DOI: | 10.21203/rs.3.rs-45442/v3 |
| Popis: | Background Voice disorders are a worldwide problem impacting human health, particularly for occupational voice users. Avoidance of surface dehydration is commonly prescribed as a protective factor against the development of dysphonia. The available literature inconclusively supports this practice and a biological mechanism for how surface dehydration of the laryngeal tissue affects voice has not been described. In this study, we used an in vivo male New Zealand white rabbit model to elucidate biological changes based on gene expression within the vocal folds from surface dehydration. Surface dehydration was induced by exposure to low humidity air (18.6% + 4.3%) for 8 h. Exposure to moderate humidity (43.0% + 4.3%) served as the control condition. Ilumina-based RNA sequencing was performed and used for transcriptome analysis with validation by RT-qPCR. Results There were 103 statistically significant differentially expressed genes identified through Cuffdiff with 61 genes meeting significance by both false discovery rate and fold change. Functional annotation enrichment and predicted protein interaction mapping showed enrichment of various loci, including cellular stress and inflammatory response, ciliary function, and keratinocyte development. Eight genes were selected for RT-qPCR validation. Matrix metalloproteinase 12 (MMP12) and macrophage cationic peptide 1 (MCP1) were significantly upregulated and an epithelial chloride channel protein (ECCP) was significantly downregulated after surface dehydration by RNA-Seq and RT-qPCR. Suprabasin (SPBN) and zinc activated cationic channel (ZACN) were marginally, but non-significantly down- and upregulated as evidenced by RT-qPCR, respectively. Conclusions The data together support the notion that surface dehydration induces physiological changes in the vocal folds and justifies targeted analysis to further explore the underlying biology of compensatory fluid/ion flux and inflammatory mediators in response to airway surface dehydration. |
| Databáze: | OpenAIRE |
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