Effect of Inflammatory Mediators on ATP Release of Human Urothelial RT4 Cells
| Autor: | Jessica R. Hughes, Kylie J Mansfield |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Article Subject lcsh:Medicine Bradykinin Inflammation Models Biological General Biochemistry Genetics and Molecular Biology Cell Line chemistry.chemical_compound Adenosine Triphosphate Internal medicine medicine Humans Urothelium General Immunology and Microbiology lcsh:R Urinary Bladder Diseases Interstitial cystitis General Medicine medicine.disease Endocrinology chemistry Overactive bladder Serotonin Inflammation Mediators medicine.symptom Urinary bladder disease Histamine Research Article |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/182862 |
| Popis: | Inflammation is an important contributor to the aetiology of a number of bladder dysfunctions including interstitial cystitis, painful bladder syndrome, and overactive bladder. The aim of this study was to examine the effects of inflammatory mediators on urothelial ATP release. Human urothelial RT4 cells were exposed to normal buffer or varying concentrations of inflammatory mediators (bradykinin, histamine, and serotonin) in the presence or absence of hypotonic stretch stimuli (1 : 2 dilution of Krebs-Henseleit buffer). Others have demonstrated that bradykinin increased stretch-induced ATP release; however, we observed no change in control or stretch-induced ATP release with bradykinin. Pretreatment of RT4 cells with histamine or serotonin decreased stretch-induced ATP release (P=0.037,P=0.040, resp.). Previous studies have demonstrated increased ATP release in response to inflammation utilising whole bladder preparations in contrast to our simple model of cultured urothelial cells. The current study suggests that it is unlikely that there is a direct interaction between the release of inflammatory mediators and increased ATP release, but rather more complex interactions occurring in response to inflammation that lead to increased bladder sensation. |
| Databáze: | OpenAIRE |
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