Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship
| Autor: | Emmanuel Garcion, José Luis Cenis, Mathie Najberg, Céline Bouré, Frank Boury, Rodolfo Molina-Peña, Carmen Alvarez-Lorenzo, Muhammad Haji Mansor, Théodore Taillé |
|---|---|
| Přispěvatelé: | Design and Application of Innovative Local Treatments in Glioblastoma (CRCINA-ÉQUIPE 17), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Instituto Murciano de Investigación y desarrollo Agrario y Alimentario (IMIDA), Universidade de Santiago de Compostela [Spain] (USC ), Bernardo, Elizabeth, Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Polymers and Plastics
Scanning electron microscope Lysine Fibroin Silk fibroin Biocompatible Materials [SDV.CAN]Life Sciences [q-bio]/Cancer 02 engineering and technology 010402 general chemistry 01 natural sciences Miscibility Mice chemistry.chemical_compound [SDV.CAN] Life Sciences [q-bio]/Cancer Porous scaffold Hyaluronic acid Materials Chemistry medicine Animals Hyaluronic Acid Aerogel sponges Tissue Engineering Tissue Scaffolds Heparin Chemistry Organic Chemistry Aerogel Biodegradation 021001 nanoscience & nanotechnology Chemokine CXCL12 0104 chemical sciences 3. Good health Brain biomimicry NIH 3T3 Cells Swelling medicine.symptom Fibroins 0210 nano-technology Gels Porosity SDF-1α controlled release Nuclear chemistry |
| Zdroj: | Carbohydrate Polymers Carbohydrate Polymers, Elsevier, 2020, 237, pp.116107. ⟨10.1016/j.carbpol.2020.116107⟩ Carbohydrate Polymers, 2020, 237, pp.116107. ⟨10.1016/j.carbpol.2020.116107⟩ |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2020.116107⟩ |
| Popis: | International audience; This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues). Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions. Series of freeze-dried sponges were prepared by associating and cross-linking within the same 3D structure, HA, SF, poly-L-lysine (PLL) and heparin (hep). Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus). Addition of SF yielded sponges with slower biodegradation. SF-HA and SF-HA-hep sponges retained 75 % and 93 % of the SDF-1α respectively after 7 days and were found to be cytocompatible in vitro. |
| Databáze: | OpenAIRE |
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