Requirement for Abasic Endonuclease Gene Homologues in Arabidopsis Seed Development
| Autor: | Mark F. Belmonte, James Hatteroth, Terence M. Murphy, Stephanie Shu, Anne B. Britt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Heterozygote DNA Plant Genotype Mutant Arabidopsis lcsh:Medicine Plant Biology Genes Plant 01 natural sciences Models Biological 03 medical and health sciences Endonuclease Plant Biology/Plant Genetics and Gene Expression Plant Biology/Plant Growth and Development Gene Expression Regulation Plant AP site Insertion lcsh:Science Gene Crosses Genetic 030304 developmental biology Genetics 0303 health sciences Multidisciplinary biology Models Genetic Arabidopsis Proteins lcsh:R Wild type Life Sciences Base excision repair biology.organism_classification Endonucleases Plants Genetically Modified Mutation Seeds biology.protein Pollen lcsh:Q 010606 plant biology & botany Research Article |
| Zdroj: | PLoS ONE Murphy, Terence M.; Belmonte, Mark; Shu, Stephanie; Britt, Anne B.; & Hatteroth, James. (2009). Requirement for Abasic Endonuclease Gene Homologues in Arabidopsis Seed Development. PLOS One, 4(1), 1-7. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/6r0836ms PLoS ONE, Vol 4, Iss 1, p e4297 (2009) |
| ISSN: | 1932-6203 |
| Popis: | Arabidopsis thaliana has three genes, Ape1L, Ape2, and Arp, that show homology to abasic (apurinic/apyrimidinic) endonuclease genes of bacterial, yeast, or animal cells. In bacteria, yeast, and animals, abasic endonucleases function in base excision repair of oxidized and other modified DNA bases. Here we report that plants with knock-out mutations in any one of Ape1L, Ape2, or Arp show no apparent differences from wild type in growth rate, growth habit, and fertility. However, coincident knock-out mutations in Ape1L and Ape2 are lethal and lead to abortion of developing embryos. Mutations of Arp are not deleterious, even in combination with one of the other two mutations. The results are consistent with the interpretation that the process of base excision repair, involving at least one intact copy of Ape1L or Ape2, is required in the process of embryogenesis. |
| Databáze: | OpenAIRE |
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