D-Galactose Causes Motor Coordination Impairment, and Histological and Biochemical Changes in the Cerebellum of Rats
| Autor: | Carlos Alexandre Netto, Angela T. S. Wyse, Bruna Zanotto, André Felipe Rodrigues, Vinícius Tejada Nunes, Eduardo Farias Sanches, Paula Pierozan, Débora Delwing Dal Magro, Helena Biasibetti, Vanusa Manfredini, Felipe Schmitz |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Cerebellum medicine.medical_specialty Ataxia Neuroscience (miscellaneous) Cell Count Nerve Tissue Proteins Carbohydrate metabolism Motor Activity 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Internal medicine Glial Fibrillary Acidic Protein medicine Animals Sulfhydryl Compounds Rats Wistar Cell damage Injections Intraventricular Caspase 3 Brain-Derived Neurotrophic Factor Galactosemia Galactose Antigens Nuclear medicine.disease Acetylcholinesterase Glutathione Motor coordination 030104 developmental biology medicine.anatomical_structure Endocrinology Neurology chemistry Biochemistry medicine.symptom 030217 neurology & neurosurgery DNA Damage |
| Zdroj: | Molecular neurobiology. 54(6) |
| ISSN: | 1559-1182 |
| Popis: | Classical galactosemia is an inborn error of carbohydrate metabolism in which patients accumulate high concentration of galactose in the brain. The most common treatment is a galactose-restricted diet. However, even treated patients develop several complications. One of the most common symptoms is motor coordination impairment, including affected gait, balance, and speech, as well as tremor and ataxia. In the present study, we investigated the effects of intracerebroventricular galactose administration on motor coordination, as well as on histological and biochemical parameters in cerebellum of adult rats. Wistar rats received 5 μL of galactose (4 mM) or saline by intracerebroventricular injection. The animals performed the beam walking test at 1 and 24 h after galactose administration. Histological and biochemical parameters were performed 24 h after the injections. The results showed motor coordination impairment at 24 h after galactose injection. Galactose also decreased the number of cells in the molecular and granular layers of the cerebellum. The immunohistochemistry results suggest that the cell types lost by galactose are neurons and astrocytes in the spinocerebellum and neurons in the cerebrocerebellum. Galactose increased active caspase-3 immunocontent and acetylcholinesterase activity, decreased acetylcholinesterase immunocontent, glutathione, and BDNF levels, as well as caused protein and DNA damage. Our results suggest that galactose induces histological and biochemical changes in cerebellum, which can be associated with motor coordination impairment. |
| Databáze: | OpenAIRE |
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