Platelet Production Time in Surgery
| Autor: | B. E. Roberts, M. J. Mcmahon, B. D. Griffin, I. E. Burton |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Time Factors Thiobarbituric acid medicine.drug_class Modified method chemistry.chemical_compound Malondialdehyde Platelet production medicine Humans Platelet Incubation Aged Aspirin Anticoagulant Hematology Middle Aged Surgery chemistry Surgical Procedures Operative Female medicine.drug |
| Zdroj: | Scandinavian Journal of Haematology. 25:127-133 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1981.tb01377.x |
| Popis: | A study of platelet production time in major surgery is described. This was equated to the time taken for renewal of maximum malondialdehyde (MDA) activity in blood platelets following suppression by a single dose of aspirin (Stuart et al 1975). The method was simplified by using EDTA disodium salt as an anticoagulant instead of citrate, resuspending platelets with small bar magnets, and reducing time of incubation with thiobarbituric acid reagent from 1 h to 15 min. Results for maximum platelet renewal taken to be the time to achieve a pre-aspirin baseline level were median in surgery 6.3 (3.8–9.2) d and controls 7.0 (6.2–12.5) d. Using the modified method, average baseline malondialdehyde (MDA) levels were 6.2 ± 1.4 nmol/109 platelets in surgical patients and 6.34 ± 1.2 nmol/109 platelets in controls. Platelet production time determination using this method is not strictly analogous compared with survival of 51Cr-labelled platelets and it however warrants further study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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