Die-off of plant pathogenic bacteria in tile drainage and anoxic water from a managed aquifer recharge site

Autor: Eisfeld, Carina, van der Wolf, Jan M., van Breukelen, Boris M., Medema, Gertjan, Velstra, Jouke, Schijven, Jack F., Hydrogeology, Environmental hydrogeology
Přispěvatelé: Hydrogeology, Environmental hydrogeology
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Agricultural Irrigation
Epidemiology
Microorganism
Pectobacterium carotovorum
Plant Science
010501 environmental sciences
Pathology and Laboratory Medicine
01 natural sciences
Vegetables
Medicine and Health Sciences
Groundwater
Ralstonia solanacearum
Multidisciplinary
geography.geographical_feature_category
biology
Plant Bacterial Pathogens
Eukaryota
food and beverages
Agriculture
Genomics
Plants
Agricultural Methods
Anoxic waters
Bacterial Pathogens
Chemistry
Medical Microbiology
Tile drainage
Physical Sciences
Medicine
Pathogens
Water Microbiology
Microcosm
Potato
Research Article
Science
Plant Pathogens
Aquifer
Context (language use)
Microbial Genomics
Solanum
Microbiology
03 medical and health sciences
Biointeractions and Plant Health
Surface Water
Genetics
Life Science
General
Microbial Pathogens
0105 earth and related environmental sciences
geography
Nitrates
Chemical Compounds
Organisms
Biology and Life Sciences
Plant Pathology
biology.organism_classification
030104 developmental biology
Agronomy
Medical Risk Factors
Earth Sciences
Environmental science
Microbiome
Hydrology
Zdroj: PLoS ONE, 16(5)
PLoS ONE, Vol 16, Iss 5, p e0250338 (2021)
PLoS One, 16(5), 1. Public Library of Science
PLoS ONE 16 (2021) 5
PLoS ONE
ISSN: 1932-6203
Popis: Managed aquifer recharge (MAR) can provide irrigation water and overcome water scarcity in agriculture. Removal of potentially present plant pathogens during MAR is essential to prevent crop diseases. We studied the die-off of three plant pathogenic bacteria in water microcosms with natural or filtered tile drainage water (TDW) at 10 and 25°C and with natural anoxic aquifer water (AW) at 10°C from a MAR site. These bacteria were: Ralstonia solanacearum (bacterial wilt), and the soft rot Pectobacteriaceae (SRP) Dickeya solani and Pectobacterium carotovorum sp. carotovorum (soft rot, blackleg). They are present in surface waters and cause destructive crop diseases worldwide which have been linked to contaminated irrigation water. Nevertheless, little is known about the survival of the SRP in aqueous environments and no study has investigated the persistence of R. solanacearum under natural anoxic conditions. We found that all bacteria were undetectable in 0.1 mL samples within 19 days under oxic conditions in natural TDW at 10°C, using viable cell counting, corresponding to 3-log10 reduction by die-off. The SRP were no longer detected within 6 days at 25°C, whereas R. solanacearum was detectable for 25 days. Whereas in anoxic natural aquifer water at 10°C, the bacterial concentrations declined slower and the detection limit was reached within 56 days. Finally, we modelled the inactivation curves with a modified Weibull model that can simulate different curve shapes such as shoulder phenomena in the beginning and long tails reflecting persistent bacterial populations. The non-linear model was shown to be a reliable tool to predict the die-off of the analysed plant pathogenic bacteria, suggesting its further application to other pathogenic microorganisms in the context of microbial risk assessment.
Databáze: OpenAIRE
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