Key Genes of Lipid Metabolism and WNT-Signaling Are Downregulated in Subcutaneous Adipose Tissue with Moderate Weight Loss
| Autor: | Tilman Kühn, Mirja Grafetstätter, Ruth Schübel, Rudolf Kaaks, Theron Johnson, Judith Greimel, Disorn Sookthai, Mario Kratz, Cornelia M. Ulrich, Romy Kirsten |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male obesity Microarray Adipose tissue Weight Loss/genetics Transcriptome transcriptomics 0302 clinical medicine Lipid Metabolism/genetics Weight loss Gene expression Wnt Signaling Pathway Nutrition and Dietetics Middle Aged Down-Regulation/genetics adipose tissue Neoplasm Proteins Real-time polymerase chain reaction Treatment Outcome Caloric Restriction/methods Female medicine.symptom lcsh:Nutrition. Foods and food supply Stearoyl-CoA Desaturase Adult Obesity/diet therapy medicine.medical_specialty Calorie restriction Subcutaneous Fat Down-Regulation lcsh:TX341-641 030209 endocrinology & metabolism Biology Article 03 medical and health sciences SDG 3 - Good Health and Well-being Internal medicine Weight Loss medicine Neoplasm Proteins/metabolism Humans Wnt Signaling Pathway/genetics Aged Caloric Restriction Membrane Proteins Lipid metabolism Lipid Metabolism Stearoyl-CoA Desaturase/metabolism 030104 developmental biology Endocrinology Cross-Sectional Studies gene expression Membrane Proteins/metabolism Subcutaneous Fat/metabolism Food Science |
| Zdroj: | Nutrients Volume 11 Issue 3 Nutrients, Vol 11, Iss 3, p 639 (2019) Schübel, R, Sookthai, D, Greimel, J, Johnson, T S, Grafetstätter, M E, Kirsten, R, Kratz, M, Ulrich, C M, Kaaks, R & Kühn, T 2019, ' Key Genes of Lipid Metabolism and WNT-Signaling Are Downregulated in Subcutaneous Adipose Tissue with Moderate Weight Loss ', Nutrients, vol. 11, no. 3 . https://doi.org/10.3390/nu11030639 |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu11030639 |
| Popis: | Smaller cross-sectional studies and bariatric surgery trials suggest that weight loss may change the expression of genes in adipose tissue that have been implicated in the development of metabolic diseases, but well-powered intervention trials are lacking. In post hoc analyses of data from a 12-week dietary intervention trial initially designed to compare metabolic effects of intermittent vs. continuous calorie restriction, we analyzed the effects of overall weight loss on the subcutaneous adipose tissue (SAT) transcriptome. Changes in the transcriptome were measured by microarray using SAT samples of 138 overweight or obese individuals (age range: 35&ndash 65 years, BMI range: 25&ndash 40, non-smokers, non-diabetics). Participants were grouped post hoc according to the degree of their weight loss by quartiles (average weight loss in quartiles 1 to 4: 0%, &minus 3.2%, &minus 5.9%, and &minus 10.7%). Candidate genes showing differential expression with weight loss according to microarray analyses were validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR), and fold changes (FCs) were calculated to quantify differences in gene expression. A comparison of individuals in the highest vs. the lowest weight loss quartile revealed 681 genes to be differentially expressed (corrected p < 0.05), with 40 showing FCs of at least 0.4. Out of these, expression changes in secreted frizzled-related protein 2 (SFRP2, FC = 0.65, p = 0.006), stearoyl-CoA desaturase (SCD, FC = &minus 1.00, p < 0.001), and hypoxia inducible lipid droplet-associated (HILPDA, FC = &minus 0.45, p = 0.001) with weight loss were confirmed by RT-qPCR. Dietary weight loss induces significant changes in the expression of genes implicated in lipid metabolism (SCD and HILPDA) and WNT-signaling (SFRP2) in SAT. |
| Databáze: | OpenAIRE |
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