Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis
| Autor: | Rita de Cassia Soler, José Angelo Lauletta Lindoso, Sara Macente Boni, Luiza Keiko Oyafuso |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology lcsh:Arctic medicine. Tropical medicine Diagnostic methods Tegumentary leishmaniasis lcsh:RC955-962 Biopsy 030231 tropical medicine 030106 microbiology Leishmaniasis Cutaneous Biology Sensitivity and Specificity Polymerase Chain Reaction Leishmania braziliensis law.invention Specimen Handling 03 medical and health sciences 0302 clinical medicine law medicine Swab Humans Polymerase chain reaction Skin Tests Tegumentary Leishmaniasis Noninvasive sampling medicine.diagnostic_test DNA Kinetoplast Reproducibility of Results Leishmaniasis General Medicine DNA Protozoan medicine.disease biology.organism_classification Infectious Diseases Histopathology Original Article |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e38 Revista do Instituto de Medicina Tropical de São Paulo; v. 59 (2017); e38 Revista do Instituto de Medicina Tropical de São Paulo Instituto de Medicina Tropical (IMT) instacron:IMT Revista do Instituto de Medicina Tropical de São Paulo, Volume: 59, Article number: e38, Published: 01 JUN 2017 Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 |
| ISSN: | 1678-9946 0036-4665 |
| Popis: | Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome of the tests performance on swab samples was compatible with PCR results on biopsy samples. The findings have also shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity of the tests and the fact that the sampling method using swabs affords greater patient comfort and safety, it could be said that this method is a promising alternative to conventional biopsy-based methods for the molecular diagnosis of leishmaniasis. |
| Databáze: | OpenAIRE |
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