Characterization of normal human embryo cells grown to over 100 population doublings
| Autor: | R. F. Schuman, R. J. Pienta, J. A. Poiley |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
education.field_of_study
Cell division Population Caseins Embryo Plant Science Neoplasms Experimental Biology Embryo Mammalian Embryonic stem cell Molecular biology Cell Line Culture Media Blood Cell culture Casein Cricetinae Karyotyping Animals Humans education Developmental biology Fetal bovine serum Cell Division Biotechnology |
| Zdroj: | In vitro. 14(5) |
| ISSN: | 0073-5655 |
| Popis: | Normal human embryonic cells were subcultured for over 100 population doublings without modification of the basic medium. The cells were evaluated for growth rate, confluent density, chromosome stability, growth in soft agar, ability to hydrolyze casein and tumorigenicity. The cells possessed the characteristics of normal cells. The batch of serum used to supplement the medium was found to be of primary importance in the long-term growth of this cell culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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