LncRNA CASC2 inhibited the viability and induced the apoptosis of hepatocellular carcinoma cells through regulating miR‐24‐3p
| Autor: | Fei Zeng, Ji-Chang Fan, Yi-Guan Le, Lin Xin |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Carcinoma Hepatocellular Mice Nude Apoptosis Biology medicine.disease_cause Biochemistry Mice 03 medical and health sciences 0302 clinical medicine In vivo medicine Animals Humans RNA Neoplasm Viability assay Molecular Biology Mice Inbred BALB C Liver Neoplasms RNA Hep G2 Cells Cell Biology medicine.disease digestive system diseases In vitro Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Hepatocellular carcinoma Immunology Cancer research Female RNA Long Noncoding Carcinogenesis |
| Zdroj: | Journal of Cellular Biochemistry. 119:6391-6397 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.26479 |
| Popis: | Background Cancer susceptibility candidate 2 (CASC2), a recently discovered long non-coding RNA (lncRNA), was confirmed to play numerous roles in several human cancers. However, the involvement and concrete mechanism of CASC2 in hepatocellular carcinoma (HCC) still need to be further elucidated. Methods The relative expressions of CASC2 and miR-24-3p in HCC tissue and cell lines were determined by quantitative real-time PCR (qRT-PCR). The effects of CASC2 and miR-24-3p on HCC cells were further assessed via cell viability and apoptosis. In vivo tumorigenesis assay was performed to verify the inhibition effect of CASC2 on the tumor growth and further clarify the important role of miR-24-3p in this mechanism. Results Compared with the paired normal tissues, the relative expression of CASC2 significantly reduced in the HCC tissues, while miR-24-3p as determined by qRT-PCR obviously increased in the HCC tissues. This observation was also found in HCC cell lines. Meanwhile, the expression of CASC2 was negatively related to miR-24-3p expression in the HCC tissues (r = -0.804, p |
| Databáze: | OpenAIRE |
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