Modulation of survival motor neuron pre-mRNA splicing by inhibition of alternative 3' splice site pairing
Autor: | Sharlene R. Lim, Klemens J. Hertel |
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Rok vydání: | 2001 |
Předmět: |
Time Factors
RNA Splicing Molecular Sequence Data Exonic splicing enhancer Nerve Tissue Proteins SMN1 Biology Biochemistry Cell Line Exon medicine Tumor Cells Cultured Humans RNA Messenger Cyclic AMP Response Element-Binding Protein Molecular Biology Alleles Genetics Splice site mutation Base Sequence Dose-Response Relationship Drug Models Genetic Intron RNA-Binding Proteins SMN Complex Proteins Cell Biology Spinal muscular atrophy Exons Oligonucleotides Antisense medicine.disease Survival of Motor Neuron 1 Protein Stop codon Introns Survival of Motor Neuron 2 Protein Protein Biosynthesis RNA splicing |
Zdroj: | The Journal of biological chemistry. 276(48) |
ISSN: | 0021-9258 |
Popis: | Spinal muscular atrophy is caused by the loss of functional survival motor neuron (SMN1) alleles. A translationally silent nucleotide transition in the duplicated copy of the gene (SMN2) leads to exon 7 skipping and expression of a nonfunctional gene product. It has been suggested that differential SMN2 splicing is caused by the disruption of an exonic splicing enhancer. Here we show that the single nucleotide difference reduces the intrinsic strength of the 3' splice site of exon 7 2-fold, whereas the strength of the 5' splice site of the exon 7 is not affected. Thus, a decrease in splice site strength is magnified in the context of competing exons. These data suggest that lower levels of exon 7 definition not only reduce intron 6 removal but, more importantly, increase the efficiency of the competing exon 7 skipping pathway. Antisense oligonucleotides were tested to modulate exon 7 inclusion, which contains the authentic translation stop codon. Oligonucleotides directed toward the 3' splice site of exon 8 were shown to alter SMN2 splicing in favor of exon 7 inclusion. These results suggest that antisense oligonucleotides could be used as a therapeutic strategy to counteract the progression of SMA. |
Databáze: | OpenAIRE |
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