Malignant transformation of human fibroblast cell strain MSU-1.1 by (+-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo [a]pyrene
| Autor: | J. Justin McCormick, Calvert Louden, Suzanne K. Kohler, Dajun Yang, Veronica M. Maher, David S. Reinhold |
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| Rok vydání: | 1992 |
| Předmět: |
Adoptive cell transfer
7 8-Dihydro-7 8-dihydroxybenzo(a)pyrene 9 10-oxide Cell Alpha (ethology) Biology Cell Line Malignant transformation chemistry.chemical_compound Cell Adhesion medicine Humans Growth Substances Fibroblast Mitosis Chromosome Aberrations Multidisciplinary Fibroblasts Molecular biology In vitro Chromosome Banding Cell Transformation Neoplastic medicine.anatomical_structure Benzo(a)pyrene chemistry Immunology Calcium Cell Division Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:2237-2241 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.89.6.2237 |
| Popis: | Treatment of MSU-1.1 cells, a near-diploid, karyotypically stable, infinite life-span human fibroblast strain, with (+-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene induced focus formation. Eight independent foci were isolated and the cell strains developed from them were examined for characteristics of malignant cells. Each grew to a higher density in medium containing 1% serum than did the MSU-1.1 cells. Three of the eight grew rapidly in serum-free medium without added growth factors, formed colonies in agarose with diameters of greater than or equal to 120 microns at a frequency of 5-19%, exhibited loss of genetic material, and, when injected into athymic mice, formed sarcomas that reached 6 mm in diameter within 2-3 wk. One produced high-grade sarcomas (progressively growing, invasive tumors exhibiting high mitotic activity); the other two produced low-grade sarcomas (tumors with a lower degree of mitotic activity) that developed focal areas of high-grade malignant cells if left in the animals for greater than 4 wk. A fourth cell strain formed high-grade sarcomas only after 2.5-3 mo, but the tumor-derived cells analyzed showed the same growth properties as the three malignant cell strains described above, exhibited loss of genetic material, and, when reinjected into athymic mice, produced high-grade sarcomas with a short latency period. |
| Databáze: | OpenAIRE |
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