Role of RIG-I, MDA-5, and PKR on the Expression of Inflammatory Chemokines Induced by Synthetic dsRNA in Airway Epithelial Cells
| Autor: | Satoshi Matsukura, Koushi Ieki, Mio Kawaguchi, Shintaro Suzuki, Shin Watanabe, Tetsuya Homma, Hiroko Takeuchi, Miho Odaka, Hideki Kuga, Masatsugu Kurokawa, Kyoko Nohtomi, Mitsuru Adachi, Fumio Kokubu |
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| Rok vydání: | 2007 |
| Předmět: |
Chemokine
Interferon-Induced Helicase IFIH1 viruses Immunology Bronchi Enzyme-Linked Immunosorbent Assay Receptors Cell Surface Inflammation Biology Polymerase Chain Reaction DEAD-box RNA Helicases eIF-2 Kinase RNA interference medicine Humans Immunology and Allergy RNA Messenger RNA Small Interfering Receptors Immunologic DEAD Box Protein 58 Chemokine CCL5 Cell Line Transformed RNA Double-Stranded RIG-I Interleukin-8 Chloroquine Epithelial Cells General Medicine Protein kinase R Toll-Like Receptor 3 Chemokine CXCL10 RNA silencing Poly I-C Cell culture biology.protein RNA Interference medicine.symptom Chemokines CXC |
| Zdroj: | International Archives of Allergy and Immunology. 143:80-83 |
| ISSN: | 1423-0097 1018-2438 |
| Popis: | Background: We hypothesized that synthetic double-stranded (ds)RNA may mimic viral infection and reported that dsRNA stimulates expression of inflammatory chemokines through a receptor of dsRNA Toll-like receptor (TLR) 3 in airway epithelial cells. In this study, we focused our study on the role of other receptors for dsRNA, such as retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated gene 5 (MDA-5), and double-stranded RNA-dependent protein kinase (PKR). Methods: Airway epithelial cell BEAS-2B was cultured in vitro. Expression of target RNA and protein were analyzed by PCR and ELISA. To analyze the role of receptors for dsRNA, knockdown of theses genes was performed with short interfering RNA (siRNA). Results: We first investigated the effects of chloroquine, an inhibitor of lysosomal acidification, on the expression of chemokines. Preincubation with 100 µM chloroquine significantly inhibited the expression of mRNA for RANTES, IP-10, and IL-8, stimulated by poly I:C, indicating that poly I:C may react with a receptor expressed inside the cells. RIG-I, MDA-5, and PKR are supposed to be expressed inside the airway epithelial cells. However, the expression of chemokines stimulated with poly I:C was not significantly inhibited for these putative receptors in the cells which were transfected with siRNA. Conclusions: Synthetic dsRNA poly I:C stimulates the expression of inflammatory chemokines in airway epithelial cells, but the putative receptors for dsRNA such as RIG-I, MDA-5, or PKR may not play pivotal roles in this process. TLR3 may play a major role as reported previously. |
| Databáze: | OpenAIRE |
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