DNA barcoding of species ofBacopacoupled with high-resolution melting analysis
| Autor: | Suchada Sukrong, Jutharat Somnuek, Jessada Denduangboripant, Thatree Phadungcharoen, Chayapol Tungphatthong, Kornkanok Ingkaninan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology General Medicine Nucleic Acid Denaturation Thailand biology.organism_classification 01 natural sciences DNA barcoding High Resolution Melt Bacopa 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences 030104 developmental biology Botany Genetics DNA Barcoding Taxonomic Plantaginaceae Bacopa monnieri Molecular Biology Biotechnology |
| Zdroj: | Genome. 61:867-877 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2018-0059 |
| Popis: | In Thailand, there are three species of Bacopa, namely, B. monnieri, B. caroliniana, and B. floribunda. Among these species of Bacopa, B. monnieri is the only medicinal species, used for the treatment of cognitive impairment and improvement of cognitive abilities because of its bioactive constituents, bacoside A and B. However, because of the similar characteristics of these species, it is difficult to differentiate among related species, resulting in confusion during identification. For this reason, and to ensure therapeutic quality for consumers, authentication is important. In this study, the three abovementioned species of Bacopa were evaluated using barcoding coupled with high-resolution melting (Bar-HRM) analysis based on primers designed for the trnL–F sequences of the three species. The melting profiles of the trnL–F amplicons of B. caroliniana and B. floribunda were clearly different from the melting profile of the trnL–F amplicon from B. monnieri; thus, the species could be discriminated by Bar-HRM analysis. Bar-HRM was then used to authenticate commercial products in various forms. The melting curves of the six commercial samples indicated that all the tested products contained genuine B. monnieri species. This method provides an efficient and reliable authentication system for future commercial herbal products and offers a reference system for quality control. |
| Databáze: | OpenAIRE |
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