The effect of functionalized self-assembling peptide scaffolds on human aortic endothelial cell function
| Autor: | Carlos E. Semino, Elsa Genové, Colette J. Shen, Shuguang Zhang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Collagen Type IV
Materials science Amino Acid Motifs Blotting Western Biophysics Bioengineering Peptide Biocompatible Materials Microscopy Atomic Force Nitric Oxide Binding Competitive Basement Membrane Biomaterials Extracellular matrix Laminin Biomimetics Cell Adhesion Humans Cell adhesion Aorta Cell Proliferation chemistry.chemical_classification biology Cell growth Circular Dichroism Cell biology Extracellular Matrix Endothelial stem cell Lipoproteins LDL chemistry Mechanics of Materials Cell culture Ceramics and Composites biology.protein Electrophoresis Polyacrylamide Gel Endothelium Vascular Peptides Self-assembling peptide |
| Zdroj: | Biomaterials. 26(16) |
| ISSN: | 0142-9612 |
| Popis: | A class of designed self-assembling peptide nanofiber scaffolds with more than 99% water content has been shown to be a good biological material for cell culture. Here, we report the functionalization of one of these peptide scaffolds, RAD16-I (AcN-RADARADARADARADA-CONH2), by direct solid phase synthesis extension at the amino terminal with three short-sequence motifs. These motifs are present in two major protein components of the basement membrane, laminin 1 (YIGSR, RYVVLPR) and collagen IV (TAGSCLRKFSTM). These motifs have been previously shown to promote specific biological activities including endothelial cell adhesion, spreading, and tubular formation. Therefore, the generic functionalized peptide developed was AcN-X-GG-RADARADARADARADA-CONH2 with each motif represented by "X". We show in this work that these tailor-made peptide scaffolds enhance the formation of confluent cell monolayers of human aortic endothelial cells (HAEC) in culture. Moreover, additional assays designed to evaluate endothelial cell function showed that HAEC monolayers obtained on these scaffolds not only maintained LDL uptake activity but also enhanced nitric oxide release and elevated laminin 1 and collagen IV deposition. These results suggest that this new scaffold provide a better physiological substrate for endothelial cell culture and suggest its further application for biomedical research, cancer biology and regenerative biology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect