In vitro plant regeneration and jasmonic acid induced bioactive chemical accumulations in two Hypericum species from Turkey
| Autor: | Cüneyt Çirak, Liudas Ivanauskas, Jolita Radušienė, E.S. Kurtarc, Mindaugas Marksa |
|---|---|
| Přispěvatelé: | OMÜ, Selçuk Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Kurtarc, E. S. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Hyperforin Hypericin Plant Science Biology 01 natural sciences chemistry.chemical_compound Murashige and Skoog medium Botany Jasmonic acid fungi Hypericum spp food and beverages Elicitation In vitro culture biology.organism_classification Phenolic compounds 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Germination Seedling Shoot Growth regulators 010606 plant biology & botany Explant culture |
| Popis: | WOS: 000506413200037 The aim of the study was to introduce two endangered species from Turkish flora, Hypericum aviculariifolium and H. pruinatum, into in vitro culture and to determine the elicitation effect of jasmonic acid (JA) on the accumulation of secondary metabolites in vitro regenerated flowering plants. A Murashige and Skoog (MS) medium supplemented with plant growth regulators were used for seed germination and seedling culture. Seedling shoot explants in MS medium supplemented with combination of 1 mg/l BA and IAA resulted in maximum and simultaneous induction of roots and shoots. The regenerants of both species survived (98%) in greenhouse cultivation and were assayed for bioactive compounds. The content of secondary metabolites of flowering plants, originating from in vitro culture and treated with different doses of JA was higher than that of intact, i.e. maternal plants providing seeds and control plants. However, the intact and control plants did not differ significantly in the amounts of the bioactive compounds tested. The chemical profile of both species was similar with quantitative differences of individual compounds. Successful establishment of a highly efficient in vitro multiplication protocol in the present study offers a crucial in vitro strategy for the further conservation of endemic and rare species and enhanced production of valuable secondary metabolites. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|