A Polymerase Chain Reaction-Based Diagnostic to Identify Larvae and Eggs of Container Mosquito Species from the Australian Region

Autor:	Beebe, NW, Whelan, P, van den Hurk, A, Ritchie, SA, Corcoran, S, Cooper, RD
Rok vydání:	2007
Předmět:	Infectious Diseases General Veterinary Tropical Medicine Insect Science fungi Parasitology
Zdroj:	Journal of Medical Entomology. 44:376-380
ISSN:	1938-2928 0022-2585
DOI:	10.1093/jmedent/44.2.376
Popis:	Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is Concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedcs aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resutled in the development of a polymarase chain reaction diagnostic procedure that uses a restriction enzyme t cut the internal transcribed scaper region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 weeks of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f4497776a1d13abb308cbc165e4f331 https://doi.org/10.1093/jmedent/44.2.376 Zobrazit plný text záznamu