Antibody class capture assays for varicella-zoster virus
| Autor: | Nathalie J. Schmidt, K W Dupuis, Bagher Forghani, C K Myoraku |
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| Rok vydání: | 1984 |
| Předmět: |
Microbiology (medical)
Immunoglobulin A Herpesvirus 3 Human viruses Fluorescent Antibody Technique Immunoglobulins medicine.disease_cause Antibodies Viral Herpes Zoster Virus Immunoglobulin G Chickenpox medicine Humans biology integumentary system business.industry Varicella zoster virus Antibody titer virus diseases Antibodies Monoclonal biochemical phenomena metabolism and nutrition medicine.disease Virology eye diseases Immunoglobulin M Immunology biology.protein Immunologic Techniques Antibody business Research Article |
| Zdroj: | Journal of clinical microbiology. 19(5) |
| ISSN: | 0095-1137 |
| Popis: | Pooled monoclonal antibodies to varicella-zoster virus (VZV) were used as "detector" antibodies in a four-phase enzyme immunofluorescence assay for determination of immunoglobulin M (IgM), IgA, and IgG antibodies to VZV. Polyclonal antisera specific for heavy chains of human IgM, IgA, and IgG were employed as "capture" antibodies on the solid phase. The antibody class capture assay (ACCA) for VZV IgM antibody detected high titers of virus-specific IgM in all patients with varicella and in 5 of 10 zoster patients. VZV IgM antibody was not detected in patients with primary herpes simplex virus infections or in other individuals without active VZV infection, with one exception, a patient with encephalitis who had other serological findings compatible with a reactivated VZV infection. VZV-specific IgA and IgG antibody titers demonstrable by ACCA were compared with those measured by solid-phase indirect enzyme immunofluorescence assay (EIFA). VZV IgA antibody titers detected in patients with varicella and zoster were variable and could not be considered to be reliable markers of active VZV infection. IgA antibody titers detected by ACCA tended to be higher than those demonstrated by solid-phase indirect EIFA in varicella and zoster patients. VZV IgG antibody titers detected by ACCA in patients with varicella, and to a lesser extent in zoster patients, were as high as or higher than those demonstrated by solid-phase indirect EIFA. However, ACCA was totally insensitive in detecting VZV IgG antibody in individuals with past infections with VZV and would not be a suitable approach for determination of immunity status to VZV. |
| Databáze: | OpenAIRE |
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