Simultaneous analysis of neutral monosaccharides, fatty acids and cholesterol as biomarkers from a drop of blood
| Autor: | Luminiţa Pilat, Cristian I Ciucanu, Eugen Sisu, Victor Dumitraşcu, Ionel Ciucanu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Methylation 01 natural sciences Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Humans Monosaccharide Hydrocarbons Iodinated General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification Chromatography Chemistry Cholesterol Fatty Acids Monosaccharides 010401 analytical chemistry Reproducibility of Results General Medicine 0104 chemical sciences Medical Laboratory Technology 030104 developmental biology Enzyme Biochemistry Quantitative analysis (chemistry) Biomarkers Saponification Methyl iodide |
| Zdroj: | Bioanalysis. 8:2147-2156 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Aim: We have developed a method for simultaneous monitoring of more biomarkers from three different classes of compounds by simultaneous analysis of neutral monosaccharides, fatty acids (FAs) and cholesterol as their per-O-methylated derivatives from a drop of blood by GC–MS. This work is a development of our previous results about analysis of neutral monosaccharides from a drop of blood. Methods & results: The simultaneous per-O-methylation was obtained by methylation in one step with methyl iodide and NaOH in DMSO. The per-O-methylated derivatives were separated in one chromatogram. The quantitative analysis was reproducible for five monosaccharides, 22 FAs and cholesterol. The results of this method were compared with those of the enzymatic methods using commercial kits. Conclusion: This method can avoid the saponification of the FA methyl esters and can analyze for the first time simultaneously neutral monosaccharides, FAs and cholesterol from a drop of blood. |
| Databáze: | OpenAIRE |
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