Nuclear entry of a cGMP-dependent kinase converts transient into long-lasting olfactory adaptation
| Autor: | Bluma J. Lesch, Bi-Tzen Juang, Damien M. O'Halloran, Noelle D. L'Etoile, Jeffery Eastham-Anderson, Andrei Goga, Julia A. Kaye, Jin I. Lee, O. Scott Hamilton |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Recombinant Fusion Proteins
Models Neurological Active Transport Cell Nucleus Gene Expression Sensory system Biology Olfactory Receptor Neurons Animals Genetically Modified Cyclic GMP-Dependent Protein Kinases medicine Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Protein kinase A Cyclic GMP Genes Helminth Binding Sites Multidisciplinary CGMP binding Behavior Animal Anatomy Biological Sciences Adaptation Physiological Olfactory fatigue Sensory neuron Protein Structure Tertiary Smell medicine.anatomical_structure Odor Odorants Neuron Signal transduction Neuroscience psychological phenomena and processes Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 107:6016-6021 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | To navigate a complex and changing environment, an animal's sensory neurons must continually adapt to persistent cues while remaining responsive to novel stimuli. Long-term exposure to an inherently attractive odor causes Caenorhabditis elegans to ignore that odor, a process termed odor adaptation. Odor adaptation is likely to begin within the sensory neuron, because it requires factors that act within these cells at the time of odor exposure. The process by which an olfactory sensory neuron makes a decisive shift over time from a receptive state to a lasting unresponsive one remains obscure. In C. elegans, adaptation to odors sensed by the AWC pair of olfactory neurons requires the cGMP-dependent protein kinase EGL-4. Using a fully functional, GFP-tagged EGL-4, we show here that prolonged odor exposure sends EGL-4 into the nucleus of the stimulated AWC neuron. This odor-induced nuclear translocation correlates temporally with the stable dampening of chemotaxis that is indicative of long-term adaptation. Long-term adaptation requires cGMP binding residues as well as an active EGL-4 kinase. We show here that EGL-4 nuclear accumulation is both necessary and sufficient to induce long-lasting odor adaptation. After it is in the AWC nucleus, EGL-4 decreases the animal's responsiveness to AWC-sensed odors by acting downstream of the primary sensory transduction. Thus, the EGL-4 protein kinase acts as a sensor that integrates odor signaling over time, and its nuclear translocation is an instructive switch that allows the animal to ignore persistent odors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|