Human Invariant Natural Killer T cells possess immune-modulating functions during Aspergillus infection
Autor: | Juergen Loeffler, Anna-Lena Schmitt, Hermann Einsele, Oliver Kurzai, Maria Bouzani, Kerstin Hünniger, Antonia Beitzen-Heineke |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Antigens Differentiation T-Lymphocyte medicine.medical_treatment Antigen-Presenting Cells chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Lymphocyte Activation Aspergillus fumigatus Microbiology Flow cytometry Antigens CD1 Immunomodulation 03 medical and health sciences Immune system Antigens CD medicine Aspergillosis Humans Lectins C-Type Antigen-presenting cell Glycoproteins biology medicine.diagnostic_test hemic and immune systems General Medicine T lymphocyte biology.organism_classification Natural killer T cell Flow Cytometry 030104 developmental biology Infectious Diseases Cytokine CD1D Immunology biology.protein Cytokines Natural Killer T-Cells Antigens CD1d |
Zdroj: | Medical mycology. 54(2) |
ISSN: | 1460-2709 |
Popis: | Aspergillus fumigatus is the most common cause for invasive fungal infections, a disease associated with high mortality in immune-compromised patients. CD1d-restricted invariant natural killer T (iNKT) cells compose a small subset of T cells known to impact the immune response toward various infectious pathogens. To investigate the role of human iNKT cells during A. fumigatus infection, we studied their activation as determined by CD69 expression and cytokine production in response to distinct fungal morphotypes in the presence of different CD1d(+) antigen presenting cells using flow cytometry and multiplex enzyme-linked immunosorbent assay (ELISA). Among CD1d(+) subpopulations, CD1d(+)CD1c(+) mDCs showed the highest potential to activate iNKT cells on a per cell basis. The presence of A. fumigatus decreased this effect of CD1d(+)CD1c(+) mDCs on iNKT cells and led to reduced secretion of TNF-α, G-CSF and RANTES. Production of other Th1 and Th2 cytokines was not affected by the fungus, suggesting an immune-modulating function for human iNKT cells during A. fumigatus infection. |
Databáze: | OpenAIRE |
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