Virus-induced gene silencing in Rauwolfia species

Autor: Lucía Atehortúa, Thomas Dugé de Bernonville, Florent Lafontaine, Gaëlle Glévarec, Nathalie Giglioli-Guivarc’h, Liuda Johana Sepúlveda, Vincent Courdavault, Inês Carqueijeiro, Arnaud Lanoue, Nicolas Papon, Audrey Oudin, Benoit St-Pierre, Marc Clastre, Sébastien Besseau, Martine Courtois, Cyrielle Corbin
Přispěvatelé: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Groupe d'Etude des Interactions Hôte-Parasite (GEIHP), Université d'Angers (UA), Laboratorio de Biotecnología, Sede de Investigación Universitaria, Universidad de Antioquia, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Universidad de Antioquia = University of Antioquia [Medellín, Colombia], Université de Tours (UT)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Protoplasma
Protoplasma, Springer Verlag, 2017, 254 (4), pp.1813-1818. ⟨10.1007/s00709-017-1079-y⟩
ISSN: 0033-183X
1615-6102
Popis: Elucidation of the monoterpene indole alkaloid biosynthesis has recently progressed in Apocynaceae through the concomitant development of transcriptomic analyses and reverse genetic approaches performed by virus-induced gene silencing (VIGS). While most of these tools have been primarily adapted for the Madagascar periwinkle (Catharanthus roseus), the VIGS procedure has scarcely been used on other Apocynaceae species. For instance, Rauwolfia sp. constitutes a unique source of specific and valuable monoterpene indole alkaloids such as the hypertensive reserpine but are also well recognized models for studying alkaloid metabolism, and as such would benefit from an efficient VIGS procedure. By taking advantage of a recent modification in the inoculation method of the Tobacco rattle virus vectors via particle bombardment, we demonstrated that the biolistic-mediated VIGS approach can be readily used to silence genes in both Rauwolfia tetraphylla and Rauwolfia serpentina. After establishing the bombardment conditions minimizing injuries to the transformed plantlets, gene downregulation efficiency was evaluated at approximately a 70% expression decrease in both species by silencing the phytoene desaturase encoding gene. Such a gene silencing approach will thus constitute a critical tool to identify and characterize genes involved in alkaloid biosynthesis in both of these prominent Rauwolfia species.
Databáze: OpenAIRE
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