Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro

Autor: Sara Silvani, Matteo Tironi, Chiara Emma Campiglio, Federica Boschetti, Marina Figliuzzi, Sara Conti, Andrea Remuzzi
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Polyesters
Cell
Cell Culture Techniques
Cell morphology
Peptides
Cyclic
Biochemistry
Microbiology
Cell function
cell morphology
mechanobiology
substrate properties
lung epithelial cells
Article
Actin cytoskeleton organization
Alveolar cells
Lipopeptides
03 medical and health sciences
0302 clinical medicine
Cell Line
Tumor
medicine
Humans
Dimethylpolysiloxanes
Lung
Molecular Biology
Adaptor Proteins
Signal Transducing
A549 cell
Chemistry
Settore ING-IND/34 - Bioingegneria Industriale
YAP-Signaling Proteins
respiratory system
In vitro
QR1-502
Culture Media
Cell biology
Pulmonary Alveoli
Cytosol
030104 developmental biology
medicine.anatomical_structure
A549 Cells
Cell culture
Alveolar Epithelial Cells
030220 oncology & carcinogenesis
cell function
Microscopy
Electrochemical
Scanning
Transcription Factors
Zdroj: Biomolecules
Volume 11
Issue 5
Biomolecules, Vol 11, Iss 675, p 675 (2021)
ISSN: 2218-273X
DOI: 10.3390/biom11050675
Popis: Cell’s microenvironment has been shown to exert influence on cell behavior. In particular, matrix-cell interactions strongly impact cell morphology and function. The purpose of this study was to analyze the influence of different culture substrate materials on phenotype and functional properties of lung epithelial adenocarcinoma (A549) cells. A549 cells were seeded onto two different biocompatible, commercially available substrates: a polyester coverslip (Thermanox™ Coverslips), that was used as cell culture plate control, and a polydimethylsiloxane membrane (PDMS, Elastosil® Film) investigated in this study as alternative material for A549 cells culture. The two substrates influenced cell morphology and the actin cytoskeleton organization. Further, the Yes-associated protein (YAP) and its transcriptional coactivator PDZ-binding motif (TAZ) were translocated to the nucleus in A549 cells cultured on polyester substrate, yet it remained mostly cytosolic in cells on PDMS substrate. By SEM analysis, we observed that cells grown on Elastosil® Film maintained an alveolar Type II cell morphology. Immunofluorescence staining for surfactant-C revealing a high expression of surfactant-C in cells cultured on Elastosil® Film, but not in cells cultured on Thermanox™ Coverslips. A549 cells grown onto Elastosil® Film exhibited morphology and functionality that suggest retainment of alveolar epithelial Type II phenotype, while A549 cells grown onto conventional plastic substrates acquired an alveolar Type I phenotype.
Databáze: OpenAIRE
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