Erythrocyte Sodium Sensitivity and Eryptosis in Chronic Hemodialysis Patients
Autor: | Anna Meyring-Wösten, Stephan Thijssen, Schantel Williams, Peter Kotanko, Viktoriya Kuntsevich, Chiarra Ornillo, Samir S. Patel, Jie Ma, Israel Campos |
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Rok vydání: | 2017 |
Předmět: |
Male
lcsh:Diseases of the circulatory (Cardiovascular) system Erythrocytes medicine.medical_treatment Erythrocyte death Eryptosis 030232 urology & nephrology lcsh:RC870-923 medicine.disease_cause chemistry.chemical_compound 0302 clinical medicine lcsh:Dermatology education.field_of_study medicine.diagnostic_test General Medicine Phosphatidylserine Middle Aged End stage renal disease Salt blood test Nephrology 030220 oncology & carcinogenesis Hemodialysis Cardiology and Cardiovascular Medicine Intracellular Adult medicine.medical_specialty Population Glycocalyx 03 medical and health sciences Renal Dialysis Internal medicine medicine Humans Blood test Renal Insufficiency Chronic education Aged Sodium lcsh:RL1-803 lcsh:Diseases of the genitourinary system. Urology Endocrinology chemistry Oxidative stress lcsh:RC666-701 Apoptosis Case-Control Studies Immunology Reactive oxygen species Sodium buffer capacity |
Zdroj: | Kidney & Blood Pressure Research, Vol 42, Iss 2, Pp 314-326 (2017) |
ISSN: | 1423-0143 1420-4096 |
Popis: | Background/Aims: In hemodialysis (HD) patients the endothelial and erythrocyte glycocalyx is impaired which in turn correlates with elevated erythrocyte sodium sensitivity (ESS). Additionally, apoptotic erythrocyte death (eryptosis), characterized by phosphatidylserine (PS) exposure on the cell surface, is increased in this population. We aimed to explore the relationship of ESS and eryptosis. Methods: Blood samples were collected from 11 healthy controls and 20 chronic HD patients before and after midweek HD. ESS was quantified by the salt blood test. PS-exposure, intracellular reactive oxygen species (ROS) of erythrocytes and reticulocytes were assessed by flow cytometry. Results: Compared to controls ESS was significantly higher in HD patients preHD and did not change during treatment. The percentage of eryptotic cells did not differ between controls and patients preHD. However, eryptosis decreased during HD. ESS and eryptosis were uncorrelated, while eryptosis was positively correlated with intracellular ROS and percent reticulocytes. Conclusions: Higher ESS levels in HD patients indicate a pathologic glycocalyx. ESS and eryptosis were not correlated. The decreased eryptosis postHD may possibly be related to dialytic uremic toxin removal, but is likely multifactorial. The relationship between eryptosis and intracellular ROS warrants further research. |
Databáze: | OpenAIRE |
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