Erythrocyte Sodium Sensitivity and Eryptosis in Chronic Hemodialysis Patients

Autor: Anna Meyring-Wösten, Stephan Thijssen, Schantel Williams, Peter Kotanko, Viktoriya Kuntsevich, Chiarra Ornillo, Samir S. Patel, Jie Ma, Israel Campos
Rok vydání: 2017
Předmět:
Male
lcsh:Diseases of the circulatory (Cardiovascular) system
Erythrocytes
medicine.medical_treatment
Erythrocyte death
Eryptosis
030232 urology & nephrology
lcsh:RC870-923
medicine.disease_cause
chemistry.chemical_compound
0302 clinical medicine
lcsh:Dermatology
education.field_of_study
medicine.diagnostic_test
General Medicine
Phosphatidylserine
Middle Aged
End stage renal disease
Salt blood test
Nephrology
030220 oncology & carcinogenesis
Hemodialysis
Cardiology and Cardiovascular Medicine
Intracellular
Adult
medicine.medical_specialty
Population
Glycocalyx
03 medical and health sciences
Renal Dialysis
Internal medicine
medicine
Humans
Blood test
Renal Insufficiency
Chronic
education
Aged
Sodium
lcsh:RL1-803
lcsh:Diseases of the genitourinary system. Urology
Endocrinology
chemistry
Oxidative stress
lcsh:RC666-701
Apoptosis
Case-Control Studies
Immunology
Reactive oxygen species
Sodium buffer capacity
Zdroj: Kidney & Blood Pressure Research, Vol 42, Iss 2, Pp 314-326 (2017)
ISSN: 1423-0143
1420-4096
Popis: Background/Aims: In hemodialysis (HD) patients the endothelial and erythrocyte glycocalyx is impaired which in turn correlates with elevated erythrocyte sodium sensitivity (ESS). Additionally, apoptotic erythrocyte death (eryptosis), characterized by phosphatidylserine (PS) exposure on the cell surface, is increased in this population. We aimed to explore the relationship of ESS and eryptosis. Methods: Blood samples were collected from 11 healthy controls and 20 chronic HD patients before and after midweek HD. ESS was quantified by the salt blood test. PS-exposure, intracellular reactive oxygen species (ROS) of erythrocytes and reticulocytes were assessed by flow cytometry. Results: Compared to controls ESS was significantly higher in HD patients preHD and did not change during treatment. The percentage of eryptotic cells did not differ between controls and patients preHD. However, eryptosis decreased during HD. ESS and eryptosis were uncorrelated, while eryptosis was positively correlated with intracellular ROS and percent reticulocytes. Conclusions: Higher ESS levels in HD patients indicate a pathologic glycocalyx. ESS and eryptosis were not correlated. The decreased eryptosis postHD may possibly be related to dialytic uremic toxin removal, but is likely multifactorial. The relationship between eryptosis and intracellular ROS warrants further research.
Databáze: OpenAIRE
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