Regulatory and junctional proteins of the blood-testis barrier in human Sertoli cells are modified by monobutyl phthalate (MBP) and bisphenol A (BPA) exposure
| Autor: | André Rebelo Peixoto, André Teves A.G. de Freitas, Wellerson Rodrigo Scarano, Cristiane Figueiredo Pinho, Mariana Antunes Ribeiro, Raquel Fantin Domeniconi |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male endocrine system medicine.medical_specialty Bisphenol A (HSeC) Human Sertoli cells Phalloidin Cell Survival Phthalic Acids Biology Endocrine Disruptors Toxicology Occludin Cell Line Male reproductive function 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phenols Antigens CD Internal medicine medicine Endocrine system Humans Benzhydryl Compounds Spermatogenesis Endocrine disruptors Blood-testis barrier Blood-Testis Barrier beta Catenin Blood–testis barrier 030219 obstetrics & reproductive medicine Sertoli Cells urogenital system General Medicine Sertoli cell Cadherins Cell biology Androgen receptor 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Monobutyl phthalate Zonula Occludens-1 Protein |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1879-3177 |
| Popis: | Made available in DSpace on 2018-12-11T17:27:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-08-01 The blood-testis barrier (BTB) is responsible for providing a protected environment and coordinating the spermatogenesis. Endocrine disruptors (EDs) might lead to infertility, interfering in the BTB structure and modulation. This study aimed to correlate the actions of two EDs, monobutyl phthalate (MBP) and bisphenol A (BPA) in different periods of exposure, in a low toxicity dose to the human Sertoli cells (HSeC) and its effects on the proteins of the BTB and regulatory proteins involved in its modulation. HSeC cells were exposed to MBP (10 μM) and BPA (20 μM) for 6 and 48 h. Western Blot assay indicated that MBP was able to reduce the expression of occludin, ZO-1, N-cadherin and Androgen Receptor (AR), while BPA leads to a reduction of occludin, ZO-1, β-catenin and AR. TGF-β2 and F-actin were not modified. Phalloidin and Hematoxylin and Eosin assay revealed phenotically disruption in Sertoli cells adhesion, without changes in F-actin expression or localization. Our data suggested both EDs present potential for disrupting the structure and maintenance of the human BTB by AR dependent pathway. Institute of Biosciences UNESP Department of Anatomy Institute of Biosciences UNESP Department of Morphology Institute of Biosciences UNESP Institute of Biosciences UNESP Department of Anatomy Institute of Biosciences UNESP Department of Morphology Institute of Biosciences UNESP |
| Databáze: | OpenAIRE |
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