Silver nanoparticles effect on Artemia salina and Allium cepa organisms: influence of test dilution solutions on toxicity and particles aggregation
| Autor: | Élvio Antônio de Campos, Marjhorie Thais Meneguzzo Deon, Márcia Teresinha Veit, Laila Karoline Ferreira, Jean Carlos Bosquette de Almeida, Soraya Moreno Palácio |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Silver Health Toxicology and Mutagenesis Sodium Metal Nanoparticles chemistry.chemical_element 010501 environmental sciences Management Monitoring Policy and Law Toxicology 01 natural sciences Polyvinyl alcohol Silver nanoparticle Sodium borohydride chemistry.chemical_compound Onions medicine Animals Particle Size 0105 earth and related environmental sciences biology General Medicine biology.organism_classification Carboxymethyl cellulose Dilution 010602 entomology chemistry Particle size Artemia Artemia salina medicine.drug Nuclear chemistry |
| Zdroj: | Ecotoxicology. 30:836-850 |
| ISSN: | 1573-3017 0963-9292 |
| Popis: | The objective of this study was to evaluate the effects of AgNPs on Artemia salina and Allium cepa, evaluating the influence of the dilution solutions on the particle behavior. The AgNPs were synthesized by chemical reduction of AgNO3 (3 and 5 mmol L−1) with sodium borohydride and stabilized with PVA (polyvinyl alcohol) and CMC (sodium carboxymethyl cellulose). The toxicity of AgNPs was evaluated in Artemia salina (mortality) using Meyer’s solution as a diluent and in Allium cepa (chromosomal aberrations) using reconstituted hard water. AgNPs showed characteristic molecular absorption bands. Particles with CMC presented hydrodynamic radius between 4 and 102 nm and with PVA between 7 and 46 nm. The studied dispersions were toxic to A. salina species. Meyer’s solution, used as dilution water in the test, caused precipitation of Ag+ and also caused changes in CMC-stabilized AgNPs, changing the shape of the nanoparticles by depositing precipitates on their surface. These changes make the results of toxicity difficult to interpret. AgNPs stabilized with PVA remained unchanged. AgNPs affected cell division and caused the appearance of chromosomal aberrations on A. cepa. Higher numbers of chromosomal aberrations occurred in dispersions with smaller particle diameters (AgNPs3-PVA and AgNPs5-PVA, without dilution). In the studied conditions the dispersions were toxic to the tested organisms, the concentrations of precursors and the type of stabilizer used influenced the particle size and toxicity. In the test with A. cepa, the reconstituted hard water did not cause changes in the dispersions of AgNPs, whereas for A. salina the Meyer solution promoted aggregation of the particles and precipitation, in the dispersions stabilized with CMC, thus changing the samples. |
| Databáze: | OpenAIRE |
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