Expression, purification and structural analysis of the Pyrococcus abyssi RNA binding protein PAB1135
| Autor: | Celso Ramos, João Arg Barbosa, Carla C. Oliveira, Juliana Silva da Luz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Operon
RNase P Short Report lcsh:Medicine RNA-binding protein Biology Bioinformatics General Biochemistry Genetics and Molecular Biology RNase H lcsh:Science (General) Gene lcsh:QH301-705.5 Medicine(all) Biochemistry Genetics and Molecular Biology(all) lcsh:R Archaeoglobus fulgidus RNA General Medicine biology.organism_classification Biochemistry RNA (METABOLISMO) lcsh:Biology (General) biology.protein human activities Pyrococcus abyssi lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 3, Iss 1, p 97 (2010) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP BMC Research Notes |
| ISSN: | 1756-0500 |
| Popis: | Background The gene coding for the uncharacterized protein PAB1135 in the archaeon Pyrococcus abyssi is in the same operon as the ribonuclease P (RNase P) subunit Rpp30. Findings Here we report the expression, purification and structural analysis of PAB1135. We analyzed the interaction of PAB1135 with RNA and show that it binds efficiently double-stranded RNAs in a non-sequence specific manner. We also performed molecular modeling of the PAB1135 structure using the crystal structure of the protein Af2318 from Archaeoglobus fulgidus (2OGK) as the template. Conclusions Comparison of this model has lead to the identification of a region in PAB1135 that could be involved in recognizing double-stranded RNA. |
| Databáze: | OpenAIRE |
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