Decreased activity of neutrophils in the presence of diferuloylmethane (curcumin) involves protein kinase C inhibition
Autor: | Viera Jančinová, Katarína Drábiková, Tomáš Perečko, Daniela Košt’álová, Katarína Bauerová, Radomír Nosál |
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Rok vydání: | 2009 |
Předmět: |
Adult
Male Curcumin Luminescence Neutrophils Arthritis Inflammation Biology Neutrophil Activation Proinflammatory cytokine Young Adult chemistry.chemical_compound Adenosine Triphosphate medicine Animals Humans Phosphorylation Protein Kinase C Protein kinase C Luciferases Renilla Respiratory Burst Pharmacology chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug Kinase Middle Aged medicine.disease Arthritis Experimental Molecular biology Rats Enzyme Activation Kinetics chemistry Rats Inbred Lew Luminescent Measurements Carcinogens Absolute neutrophil count Tetradecanoylphorbol Acetate medicine.symptom Oxidation-Reduction |
Zdroj: | European Journal of Pharmacology. 612:161-166 |
ISSN: | 0014-2999 |
Popis: | article i nfo Diferuloylmethane (curcumin) has been shown to act beneficially in arthritis, particularly through downregulated expression of proinflammatory cytokines and collagenase as well as through the modulated activities of T lymphocytes and macrophages. In this study its impact on activated neutrophils was investigated both in vitro and in experimental arthritis. Formation of reactive oxygen species in neutrophils was recorded on the basis of luminol- or isoluminol-enhanced chemiluminescence. Phosphorylation of neutrophil protein kinases C alpha and beta II was assessed by Western blotting, using phosphospecific antibodies. Adjuvant arthritis was induced in Lewis rats by heat-killed Mycobacterium butyricum. Diferuloylmethane or methotrexate was administered over a period of 28 days after arthritis induction. Under in vitro conditions, diferuloylmethane (1-100 µM) reduced dose-dependently oxidant formation both at extra- and intracellular level and it effectively reduced protein kinase C activation. Adjuvant arthritis was accompanied by an increased number of neutrophils in blood and by a more pronounced spontaneous as well as PMA (phorbol myristate acetate) stimulated chemiluminescence. Whereas the arthritis-related alterations in neutrophil count and in spontaneous chemiluminescence were not modified by diferuloylmethane, the increased reactivity of neutrophils to PMA was less evident in diferuloylmethane-treated animals. The effects of diferuloylmethane were comparable with those of methotrexate. Diferuloylmethane was found to be a potent inhibitor of neutrophil functions both in vitro and in experimental arthritis. As neutrophils are considered to be cells with the greatest capacity to inflict damage within diseased joints, the observed effects could represent a further mechanism involved in the antirheumatic activity of diferuloylmethane. |
Databáze: | OpenAIRE |
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