Phosphorylation of the anti-oncogene products and control of the cell cycle
| Autor: | Tetsu Akiyama, Shuji Sumida, Kumao Toyoshima, Shi-Qiong Xu, Tohru Ohuchi |
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| Rok vydání: | 1992 |
| Předmět: |
Kinase
Cyclin-dependent kinase 2 Cell Cycle General Medicine Cell cycle Biology Molecular biology DNA-binding protein Retinoblastoma Protein General Biochemistry Genetics and Molecular Biology Cell biology Cyclin D1 Mitogen-activated protein kinase biology.protein Tumor Cells Cultured Phosphorylation Humans Protein phosphorylation Protein Kinases |
| Zdroj: | The Tohoku journal of experimental medicine. 168(2) |
| ISSN: | 0040-8727 |
| Popis: | AKIYAMA, T., OHUCHI, T., SUMIDA, S., XU, S. and TOYOSHIMA, K. Phosphorylation of the Antioncogene Products and Control of the Cell Cycle. Tohoku J. Exp. Med., 1992, 168 (2), 153-157-To investigate the function of the RB protein, we have studied cellular RB binding proteins and protein kinases responsible for phosphorylation of the RB protein. (1) We purified a cellular RB- associated protein p56 which competes with SV40 large T antigen for binding to the RB protein. (2) In another experiment, we screened expression libraries of U937 monocytic leukemia cell line by West-Western method and obtained two cDNA clones that encode RB binding proteins. (3) The RB protein was found to be phosphorylated by cdk2 and MAP kinase in vitro. Most of the sites phosphorylated in vitro are the same as those phosphorylated in vivo and the time course of activation of cdk2 in the cell cycle were similar to that of phosphorylation of the RB protein.-cdk2 kinase; MAP kinase; phosphorylation; RB protein; RB-associated protein |
| Databáze: | OpenAIRE |
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