Geno-Tox: Cell Array Biochip for Genotoxicity Monitoring and Classification
| Autor: | Man Bock Gu, Joo Myung Ahn |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
DNA damage
In vitro genotoxicity DNA Recombinant Bioengineering Computational biology Biology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Genotoxicity testing fluids and secretions medicine Promoter Regions Genetic Biochip Molecular Biology Bacteria Cellular array Mutagenicity Tests General Medicine Bioluminescent bacteria Cells Immobilized Molecular biology Mechanism of action Tissue Array Analysis bacteria medicine.symptom Genetic Engineering Genotoxicity DNA Damage Mutagens Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 168:752-760 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-012-9815-4 |
| Popis: | In vitro genotoxicity tests detect carcinogens that are thought to act primarily via a mechanism involving direct genetic damage. In this study, we constructed a Geno-Tox cell array chip for genotoxicity testing; eight recombinant bioluminescent bacteria were used to successfully fabricate a Geno-Tox cell array chip. Four well-characterized DNA damage chemicals were selected to determine the capabilities of this Geno-Tox array chip, and each strain of the chip was distinctly responsive, according to the specific mode of genotoxic action. Therefore, this Geno-Tox cell array chip could be implemented to characterize and understand the genotoxic modes of impact; thus, it could be used to provide the genotoxic mechanism of action for new drugs or unknown or newly synthesized chemicals in food and the environment. |
| Databáze: | OpenAIRE |
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