Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia
| Autor: | Lela Su'ut, Kasing Apun, Lesley Maurice Bilung, Chai Fung Pui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype lcsh:Arctic medicine. Tropical medicine Surveillance data Article Subject lcsh:RC955-962 animal diseases 030106 microbiology 030231 tropical medicine Microbiology law.invention 03 medical and health sciences 0302 clinical medicine Leptospira law Leptospira noguchii Leptospira meyeri Polymerase chain reaction Leptospira wolffii biology General Medicine biology.organism_classification bacterial infections and mycoses Prevalence studies bacteria Parasitology |
| Zdroj: | Journal of Tropical Medicine, Vol 2017 (2017) |
| ISSN: | 1687-9686 |
| DOI: | 10.1155/2017/3760674 |
| Popis: | Various prevalence studies onLeptospirain animals and humans, as well as environmental samples, had been conducted worldwide, including Malaysia. However, limited studies have been documented on the presence of pathogenic, intermediate, and saprophyticLeptospirain selected animals and environments. This study was therefore conducted to detectLeptospiraspp. in rats, soil, and water from urban areas of Sarawak using the polymerase chain reaction (PCR) method. A total of 107 rats, 292 soil samples, and 324 water samples were collected from April 2014 to February 2015. PathogenicLeptospirawas present in 5.6% (6/107) of rats, 11.6% (34/292) of soil samples, and 1.9% (6/324) of water samples. IntermediateLeptospirawas present in 2.7% (8/292) of soil samples and 1.9% (6/324) of water samples. SaprophyticLeptospirawas present in 10.3% (11/107) of rats, 1.4% (4/292) of soil samples, and 0.3% (1/324) of water samples. From this study, 76Leptospiraspp. were isolated. Based on DNA sequencing, the dominantLeptospiraspp. circulating in urban areas of Sarawak are pathogenicLeptospira noguchii, intermediateLeptospira wolffiiserovar Khorat, and saprophyticLeptospira meyeri, respectively. Overall, this study provided important surveillance data on the prevalence ofLeptospiraspp. from rats and the environment, with dominant local serovars in urban areas of Sarawak. |
| Databáze: | OpenAIRE |
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