Gonadotropin-releasing hormone receptor (Gnrhr) gene knock out: Normal growth and development of sensory, motor and spatial orientation behavior but altered metabolism in neonatal and prepubertal mice

Autor: Nancy M. Sherwood, Ellen R. Busby
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Sensory Receptors
Physiology
Social Sciences
lcsh:Medicine
Biochemistry
Fat pad
Fats
Mice
Glucose Metabolism
Reflexes
Medicine and Health Sciences
Psychology
Lipid Hormones
Receptor
lcsh:Science
Mice
Knockout
Mammals
Kidney
Multidisciplinary
Behavior
Animal
Estradiol
Lipids
medicine.anatomical_structure
Adipose Tissue
Physiological Parameters
Vertebrates
Carbohydrate Metabolism
Female
Sensory Perception
Anatomy
Gonadotropin-releasing hormone receptor
Locomotion
Research Article
Signal Transduction
medicine.medical_specialty
Period (gene)
Carbohydrate metabolism
Biology
Motor Activity
Rodents
03 medical and health sciences
Insulin resistance
Ocular System
Internal medicine
Reflex
medicine
Animals
Orientation
Spatial
Body Weight
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
medicine.disease
Hormones
030104 developmental biology
Endocrinology
Metabolism
Amniotes
Eyes
lcsh:Q
Insulin Resistance
Energy Metabolism
Head
Receptors
LHRH
Hormone
Neuroscience
Zdroj: PLoS ONE, Vol 12, Iss 3, p e0174452 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Gonadotropin-releasing hormone (GnRH) is important in the control of reproduction, but its actions in non-reproductive processes are less well known. In this study we examined the effect of disrupting the GnRH receptor in mice to determine if growth, metabolism or behaviors that are not associated with reproduction were affected. To minimize the effects of other hormones such as FSH, LH and sex steroids, the neonatal-prepubertal period of 2 to 28 days of age was selected. The study shows that regardless of sex or phenotype in the Gnrhr gene knockout line, there was no significant difference in the daily development of motor control, sensory detection or spatial orientation among the wildtype, heterozygous or null mice. This included a series of behavioral tests for touch, vision, hearing, spatial orientation, locomotory behavior and muscle strength. Neither the daily body weight nor the final weight on day 28 of the kidney, liver and thymus relative to body weight varied significantly in any group. However by day 28, metabolic changes in the GnRH null females compared with wildtype females showed a significant reduction in inguinal fat pad weight normalized to body weight; this was accompanied by an increase in glucose compared with wildtype females shown by Student-Newman-Keuls Multiple Comparison test and Student's unpaired t tests. Our studies show that the GnRH-GnRHR system is not essential for growth or motor/sensory/orientation behavior during the first month of life prior to puberty onset. The lack of the GnRH-GnRHR axis, however, did affect females resulting in reduced subcutaneous inguinal fat pad weight and increased glucose with possible insulin resistance; the loss of the normal rise of estradiol at postnatal days 15-28 may account for the altered metabolism in the prepubertal female pups.
Databáze: OpenAIRE
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