A Fluorescence Kinetic-Based Aptasensor Employing Stilbene Isomerization for Detection of Thrombin
| Autor: | Kuiyan Cui, Liyan Wang, Xinling Zeng, Xinsheng Peng, Huizhi Chen, Xiaoxian Zhu, Terry W. J. Steele, Hui Xu, Qing Zhou, Yubin Zhou |
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| Přispěvatelé: | School of Materials Science and Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
Analyte Aptamer Photoisomerization Kinetics Article Thrombin medicine General Materials Science kinetic mode Detection limit Microscopy QC120-168.85 Materials [Engineering] Chemistry QH201-278.5 aptamer photoisomerization Engineering (General). Civil engineering (General) biosensors Fluorescence Combinatorial chemistry thrombin TK1-9971 Descriptive and experimental mechanics Electrical engineering. Electronics. Nuclear engineering fluorescence TA1-2040 Biosensor medicine.drug |
| Zdroj: | Materials Volume 14 Issue 22 Materials, Vol 14, Iss 6927, p 6927 (2021) |
| ISSN: | 1996-1944 |
| Popis: | It is important to detect thrombin due to its physiological and pathological roles, where rapid and simple analytical approaches are needed. In this study, an aptasensor based on fluorescence attenuation kinetics for the detection of thrombin is presented, which incorporates the features of stilbene and aptamer. We designed and synthesized an aptasensor by one-step coupling of stilbene compound and aptamer, which employed the adaptive binding of the aptamer with thrombin to cause a change in stilbene fluorescence attenuation kinetics. The sensor realized detection of thrombin by monitoring the variation in apparent fluorescence attenuation rate constant (kapp), which could be further used for probing of enzyme–aptamer binding. In comprehensive studies, the developed aptasensor presented satisfactory performance on repeatability, specificity, and regeneration capacity, which realized rapid sensing (10 s) with a limit of detection (LOD) of 0.205 μM. The strategy was successful across seven variants of thrombin aptasensors, with tunable kapp depending on the SITS (4-Acetamido-4′-isothiocyanato-2,2′-stilbenedisulfonic acid disodium salt hydrate) grafting site. Analyte detection mode was demonstrated in diluted serum, requiring no separation or washing steps. The new sensing mode for thrombin detection paves a way for high-throughput kinetic-based sensors for exploiting aptamers targeted at clinically relevant proteins. |
| Databáze: | OpenAIRE |
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