Transcription factor hlh-2/E/Daughterless drives expression of α integrin ina-1 during DTC migration in C. elegans
| Autor: | Allison P. Kann, Christopher M. Meighan, Emily R. Egress |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Integrins
Transcription Genetic Organogenesis Cell Integrin Molecular Sequence Data Motility Cell Movement Genetics medicine Transcriptional regulation Basic Helix-Loop-Helix Transcription Factors Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Transcription factor Gene knockdown biology Base Sequence Gene Expression Regulation Developmental Cell migration General Medicine biology.organism_classification Molecular biology medicine.anatomical_structure biology.protein |
| Zdroj: | Gene. 568(2) |
| ISSN: | 1879-0038 |
| Popis: | Integrins are involved in a vast number of cell behaviors due to their roles in adhesion and signaling. The regulation of integrin expression is of particular interest as a mechanism to drive developmental events and for the role of altered integrin expression profiles in cancer. Dynamic regulation of the expression of integrin receptors is required for the migration of the distal tip cell (DTC) during gonadogenesis in Caenorhabditis elegans. α integrin ina-1 is required for DTC motility, yet is up-regulated by an unknown mechanism. Analysis of the promoter for α integrin ina-1 identified two E-box sequences that are required for ina-1 expression in the DTC. Knockdown of transcription factor hlh-2, an established E-box binding partner and ortholog of E/Daughterless, prevented expression of a transcriptional fusion of the ina-1 promoter to RFP and blocked DTC migration. Similarly, knockdown of hlh-2 also prevented expression of a translational fusion of the genomic ina-1 gene to GFP while blocking DTC migration. Knockdown of HLH-2 binding partner MIG-24 also reduced ina-1 expression and DTC migration. Overall, these results show that the transcription factor hlh-2 is required for up-regulation of ina-1 at the onset of DTC migration. |
| Databáze: | OpenAIRE |
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