Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations
| Autor: | Laimdota Zizmare, Christina N. Boyle, Sabrina Buss, Sandrine Louis, Laura Kuebler, Ketki Mulay, Ralf Krüger, Lara Steinhauer, Isabelle Mack, Manuel Rodriguez Gomez, Kristina Herfert, Yvonne Ritze, Christoph Trautwein |
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| Přispěvatelé: | University of Zurich, Ritze, Yvonne |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
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Bacterial Male Sucrose QH301-705.5 1503 Catalysis Gastric Bypass microbiome 1607 Spectroscopy RYGB metabolomics inflammation gut-brain axis GABA 3-hydroxybutyrate DNA Ribosomal Catalysis Inorganic Chemistry Feces RNA Ribosomal 16S 1312 Molecular Biology 1706 Computer Science Applications Animals Metabolomics Obesity Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Phylogeny Spectroscopy Caloric Restriction Bacteria 1604 Inorganic Chemistry Organic Chemistry nutritional and metabolic diseases Sequence Analysis DNA General Medicine 10081 Institute of Veterinary Physiology Gastrointestinal Microbiome Rats Computer Science Applications Chemistry Disease Models Animal C-Reactive Protein Glucose Case-Control Studies 570 Life sciences biology 1606 Physical and Theoretical Chemistry 1605 Organic Chemistry |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1126 International Journal of Molecular Sciences, Vol 23, Iss 1126, p 1126 (2022) |
| DOI: | 10.5167/uzh-217752 |
| Popis: | Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity. Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches. We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model. After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery. RYGB, sham pair-fed, and sham ad libitum-fed groups further continued on the diet after recovery. Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery. Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling. Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk. The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver. Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action. |
| Databáze: | OpenAIRE |
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