Protective effect of Opuntia ficus - indica L. cladodes against UVA-induced oxidative stress in normal human keratinocytes

Autor: Renata Piccoli, Alba Silipo, Ganna Petruk, Andrea Bonina, Luisa Rizza, Rosa Lanzetta, Daria Maria Monti, Paola Imbimbo, Flaviana Di Lorenzo
Přispěvatelé: Petruk, Ganna, Di Lorenzo, Flaviana, Imbimbo, Paola, Silipo, Alba, Bonina, Andrea, Rizza, Luisa, Piccoli, Renata, Monti, Daria Maria, Lanzetta, Rosa
Rok vydání: 2017
Předmět:
Keratinocytes
Eucomic acid
0301 basic medicine
Antioxidant
medicine.medical_treatment
Clinical Biochemistry
Pharmaceutical Science
medicine.disease_cause
Biochemistry
Antioxidants
Piscidic acid
Lipid peroxidation
Human health
chemistry.chemical_compound
Drug Discovery
Food science
Cladode water extract
biology
Chemistry
Opuntia
04 agricultural and veterinary sciences
Glutathione
040401 food science
UVA-induced oxidative stre
Molecular Medicine
Cell Survival
Ultraviolet Rays
Opuntia ficus
Opuntia ficus-indica L
Protective Agents
Cell Line
03 medical and health sciences
0404 agricultural biotechnology
Cladodes
medicine
Humans
Molecular Biology
Beneficial effects
Plant Extracts
Drug Discovery3003 Pharmaceutical Science
Organic Chemistry
biology.organism_classification
Oxidative Stress
030104 developmental biology
Apoptosis
Reactive Oxygen Species
Oxidative stress
Zdroj: Bioorganic & Medicinal Chemistry Letters. 27:5485-5489
ISSN: 0960-894X
DOI: 10.1016/j.bmcl.2017.10.043
Popis: Opuntia ficus-indica L. is known for its beneficial effects on human health, but still little is known on cladodes as a potent source of antioxidants. Here, a direct, economic and safe method was set up to obtain water extracts from Opuntia ficus-indica cladodes rich in antioxidant compounds. When human keratinocytes were pre-treated with the extract before being exposed to UVA radiations, a clear protective effect against UVA-induced stress was evidenced, as indicated by the inhibition of stress-induced processes, such as free radicals production, lipid peroxidation and GSH depletion. Moreover, a clear protective effect against apoptosis in pre-treated irradiated cells was evidenced. We found that eucomic and piscidic acids were responsible for the anti-oxidative stress action of cladode extract. In conclusion, a bioactive, safe, low-cost and high value-added extract from Opuntia cladodes was obtained to be used for skin health/protection.
Databáze: OpenAIRE
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